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Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody

Details for Product No. ABIN269641
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Antigen
Synonyms CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM, C85791, Cd31, Pecam
Reactivity
Mouse (Murine)
(379), (183), (67), (41), (30), (20), (11), (5), (2), (1)
Host
Rat
(290), (155), (79), (4), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(55), (46), (30), (19), (9), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), In vivo Studies (in vivo), Immunoprecipitation (IP)
(294), (197), (116), (108), (98), (70), (60), (51), (36), (24), (19), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mL
Shipping to United States (Change)
Catalog No. ABIN269641
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen mouse endothelial cell line T-end
Clone MEC13-3
Isotype IgG2a kappa
Purification Protein G purified
Alternative Name CD31 / PECAM1
Background CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesionmolecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily,expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages,dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. Theprimary ligands for CD31 have been reported to be CD38 and the vitronectin receptor(á,v â,3 integrin, CD51/CD61). Other reported functions of CD31are neutrophil emigration to sites of inflammation, and angiogenesis. The MEC13.3antibody has been shown to inhibit in vitro and in vivo functions of CD31. Alternate Names: anti-CSPG6 - Chondroitin Sulfate Proteoglycan 6 antibody, anti-BAM - Bamacanantibody, anti-HCAP - Human Chromosome- Associated Polypeptide antibody.
Gene Symbol: PECAM1
Gene ID 5175
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes This CD31/PECAM1 Antibody (MEC13.3) is useful for Flow Cytometry, Immunoprecipitation, Immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results. In vivo assay was reported in scientific literature. Use in vitro assays reported in scientific literature (PMID 24647208)
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:1000-1:2000, Immunohistochemistry 1:100-1:500, Immunohistochemistry-Frozen 1:100-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunoprecipitation 1:10-1:500, In vitro assay, In vivo assay
Protocol Protocol specific for CD31 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Newman, Berndt, Gorski et al.: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, 1994 (PubMed).

Baldwin, Shen, Yan et al.: "Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development." in: Development (Cambridge, England), Vol. 120, Issue 9, pp. 2539-53, 1994 (PubMed).

Rosenblum, Murata, Nelson et al.: "Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles." in: The American journal of pathology, Vol. 145, Issue 1, pp. 33-6, 1994 (PubMed).

Vecchi, Garlanda, Lampugnani et al.: "Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium." in: European journal of cell biology, Vol. 63, Issue 2, pp. 247-54, 1994 (PubMed).

Christofidou-Solomidou, Nakada, Williams et al.: "Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4872-8, 1997 (PubMed).

DeLisser, Christofidou-Solomidou, Strieter et al.: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, 1997 (PubMed).

Voswinckel, Ziegelhoeffer, Heil et al.: "Circulating vascular progenitor cells do not contribute to compensatory lung growth." in: Circulation research, Vol. 93, Issue 4, pp. 372-9, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN269641
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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