Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody

Antigen: Platelet/endothelial Cell Adhesion Molecule (PECAM1)

Synonyms: CD31, PECAM-1, FLJ58394, Cd31, Pecam, C85791, MGC102160, MGC124998, PECAM1

Clonality: Monoclonal (MEC13-3)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN269641

Additional Information

Alternative name: CD31 / PECAM1

Immunogen: Polyoma middle T transformed EC line tEnd.1.

Cross-Reactivity: Mouse (Murine)

Clone: MEC13-3

Description: CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesionmolecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily,expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages,dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. Theprimary ligands for CD31 have been reported to be CD38 and the vitronectin receptor(á,v â,3 integrin, CD51/CD61). Other reported functions of CD31are neutrophil emigration to sites of inflammation, and angiogenesis. The MEC13.3antibody has been shown to inhibit in vitro and in vivo functions of CD31. Alternate Names: anti-CSPG6 - Chondroitin Sulfate Proteoglycan 6 antibody, anti-BAM - Bamacanantibody, anti-HCAP - Human Chromosome- Associated Polypeptide antibody.

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 0.25 ug per 10^6 cells in 100 ul volume.,immunoprecipitation

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Phosphate-buffered solution, pH 7.2. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Newman, Berndt, Gorski et al.: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, 1994 (PubMed).

Baldwin, Shen, Yan et al.: "Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development." in: Development (Cambridge, England), Vol. 120, Issue 9, pp. 2539-53, 1994 (PubMed).

Rosenblum, Murata, Nelson et al.: "Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles." in: The American journal of pathology, Vol. 145, Issue 1, pp. 33-6, 1994 (PubMed).

Vecchi, Garlanda, Lampugnani et al.: "Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium." in: European journal of cell biology, Vol. 63, Issue 2, pp. 247-54, 1994 (PubMed).

Christofidou-Solomidou, Nakada, Williams et al.: "Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4872-8, 1997 (PubMed).

DeLisser, Christofidou-Solomidou, Strieter et al.: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, 1997 (PubMed).

Voswinckel, Ziegelhoeffer, Heil et al.: "Circulating vascular progenitor cells do not contribute to compensatory lung growth." in: Circulation research, Vol. 93, Issue 4, pp. 372-9, 2003 (PubMed).