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CD31 antibody (Platelet/endothelial Cell Adhesion Molecule)

Details for Product anti-PECAM1 Antibody No. ABIN269641, Supplier: Log in to see
Antigen
  • C85791
  • CD31
  • Cd31
  • CD31/EndoCAM
  • endoCAM
  • GPIIA'
  • PECA1
  • Pecam
  • PECAM-1
Reactivity
Mouse (Murine)
808
333
133
88
69
63
22
22
17
16
16
9
5
4
1
Host
Rat
745
213
204
16
7
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD31 antibody is un-conjugated
104
92
85
37
25
24
21
20
16
15
14
14
13
13
11
10
10
10
9
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), In vivo Studies (in vivo), Immunoprecipitation (IP), Immunohistochemistry (IHC)
774
457
327
311
293
235
207
205
177
21
12
11
11
11
10
10
10
8
5
5
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen mouse endothelial cell line T-end
Clone MEC13-3
Isotype IgG2a kappa
Purification Protein G purified
Alternative Name CD31/PECAM-1 (PECAM1 Antibody Abstract)
Background Gene Symbol: PECAM1
Gene ID 5175
Application Notes Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:1000-1:2000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:100-1:500, In vitro assay, In vivo assay
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for CD31 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-CD31 antibody (Platelet/endothelial Cell Adhesion Molecule) (ABIN269641) anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibody
Immunofluorescence (IF) image for anti-CD31 antibody (Platelet/endothelial Cell Adhesion Molecule) (ABIN269641) Immunocytochemistry/Immunofluorescence: CD31/PECAM1 Antibody (MEC13.3) [ABIN269641] -...
Flow Cytometry (FACS) image for anti-CD31 antibody (Platelet/endothelial Cell Adhesion Molecule) (ABIN269641) Flow Cytometry: CD31/PECAM1 Antibody (MEC13.3) [ABIN269641] - C57BL/6 mouse splenocyt...
Product cited in: Takeda, Hossain, Rantakari, Simmons, Sasaki, Salmi, Jalkanen, Miyasaka: "Thymocytes in Lyve1-CRE/S1pr1(f/f) Mice Accumulate in the Thymus due to Cell-Intrinsic Loss of Sphingosine-1-Phosphate Receptor Expression." in: Frontiers in immunology, Vol. 7, pp. 489, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Takeuchi, Yanai, Kumase, Morizane, Suzuki, Kayama, Brodowska, Nakazawa, Miller, Connor, Vavvas: "EGF-like-domain-7 is required for VEGF-induced Akt/ERK activation and vascular tube formation in an ex vivo angiogenesis assay." in: PLoS ONE, Vol. 9, Issue 3, pp. e91849, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,In vitro Assay,Immunofluorescence

Salatino, Croci, Laderach, Compagno, Gentilini, Dalotto-Moreno, Dergan-Dylon, Méndez-Huergo, Toscano, Cerliani, Rabinovich: "Regulation of galectins by hypoxia and their relevance in angiogenesis: strategies and methods." in: Methods in molecular biology (Clifton, N.J.), Vol. 1207, pp. 293-304, 2014 (PubMed).

Voswinckel, Ziegelhoeffer, Heil, Kostin, Breier, Mehling, Haberberger, Clauss, Gaumann, Schaper, Seeger: "Circulating vascular progenitor cells do not contribute to compensatory lung growth." in: Circulation research, Vol. 93, Issue 4, pp. 372-9, 2003 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Christofidou-Solomidou, Nakada, Williams, Muller, DeLisser: "Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4872-8, 1997 (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, 1997 (PubMed). (Sample species: Mouse (Murine)). Further details: In vivo Studies

Baldwin, Shen, Yan, DeLisser, Chung, Mickanin, Trask, Kirschbaum, Newman, Albelda: "Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development." in: Development (Cambridge, England), Vol. 120, Issue 9, pp. 2539-53, 1994 (PubMed). (Sample species: Mouse (Murine)). Further details: Flow Cytometry

Rosenblum, Murata, Nelson, Werner, Ranken, Harmon: "Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles." in: The American journal of pathology, Vol. 145, Issue 1, pp. 33-6, 1994 (PubMed). (Sample species: Mouse (Murine)). Further details: In vivo Studies

Vecchi, Garlanda, Lampugnani, Resnati, Matteucci, Stoppacciaro, Schnurch, Risau, Ruco, Mantovani: "Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium." in: European journal of cell biology, Vol. 63, Issue 2, pp. 247-54, 1994 (PubMed).

Background publications DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, 1994 (PubMed).

Newman, Berndt, Gorski, White, Lyman, Paddock, Muller: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).