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Nucleolin antibody (NCL) (AA 284-709)

Details for Product anti-NCL Antibody No. ABIN269716, Supplier: Log in to see
Antigen
  • wu:fa12d03
  • zgc:152810
  • ATNUC-L2
  • ATRANGAP1
  • PARALLEL1-LIKE 1
  • PARLL1
  • RAN GTPASE-ACTIVATING PROTEIN 1
  • nucleolin like 2
  • ATNUC-L1
  • F27K7.6
  • F27K7_6
  • PARALLEL 1
  • PARL1
  • nucleolin like 1
  • Nucleolin
  • eIF3-p170
  • eif3
  • eif3-theta
  • eif3s10
  • ncl
  • tif32
  • nucl
  • LOC100282923
  • LOC100283058
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
Epitope
AA 284-709
27
21
16
15
14
9
9
7
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Plant
133
39
22
5
5
3
3
3
3
3
3
2
1
Host
Rabbit
102
37
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
11
10
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunomicroscopy (IM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
107
57
48
45
28
17
16
12
11
4
2
1
Supplier
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Immunogen A fusion protein containing amino acids 284-709 of human Nucleolin.
Purification Whole antisera
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein inactively dividing cells, whereas the degraded forms are relatively abundant innon-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. Itplays a role in rDNA transcription, organization, and rRNA processing. It may alter DNAorganization in response to cell cycle controls. It is also a good mammalian nucleolusmarker. Alternate Names: anti-nucl antibody, anti-protein C23 antibody, anti-NCL antibody, anti-Nucleolin antibody,anti-C23 antibody.
Gene Symbol: NCL
Gene ID 4691, 17975
UniProt P19338
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
Pathways
Application Notes This Nucleolin antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Electron Microscopy. Chromatin Immunoprecipitation was reported in scientific literature. By Western Blot, a band at ~106 kDa represents human Nucleolin. Heat mediated antigen retrieval is recommended for IHC-P.
Recommended dilutions: Chromatin Immunoprecipitation, Electron Microscopy 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry 1:50-1:100, Immunohistochemistry-Paraffin 1:50-1:100, Immunomicroscopy, Western Blot 1:4000
Protocol Protocol specific for Nucleolin Antibody IHC-FFPE sections I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Nucleolin antibody (NCL) (AA 284-709) (ABIN269716) In-situ immunofluorescent staining of PD31 murine pre-B cells using ABIN153181.
Western Blotting (WB) image for anti-Nucleolin antibody (NCL) (AA 284-709) (ABIN269716) anti-Nucleolin (NCL) (AA 284-709) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Nucleolin antibody (NCL) (AA 284-709) (ABIN269716) Detection of Nucleolin in human tonsil germinal centers using ABIN153181.
Product cited in: Jin, Li, Davis et al.: "Identification of novel proteins associated with both alpha-synuclein and DJ-1." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 5, pp. 845-59, 2007 (PubMed).

Huddleson, Ahmad, Lingrel: "Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15121-8, 2006 (PubMed).

Background publications Hanakahi, Dempsey, Li et al.: "Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3605-10, 1997 (PubMed).