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FOXP3 antibody (Forkhead Box P3) (C-Term)

Details for Product anti-FOXP3 Antibody No. ABIN269718, Supplier: Login to see New
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Synonyms foxp3, FOXP3, AIID, DIETER, IPEX, PIDX, XPID, JM2, scurfin, sf, RGD1562112
C-Term, AA 400-431
(98), (23), (14), (13), (13), (13), (11), (6), (6), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(343), (192), (61), (24), (24), (23), (16), (12), (9), (8), (2), (2), (1), (1)
(220), (135), (71), (35)
This FOXP3 antibody is un-conjugated
(37), (30), (20), (19), (15), (14), (13), (10), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
(248), (172), (149), (90), (42), (38), (32), (30), (19), (19), (17), (6), (5), (5), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 25 μL
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Immunogen A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400-431).
Cross-Reactivity (Details) This antibody does not appear to detect the mouse FOXP3 protein by western blot, but does work on mouse tissue for IHC (see data below).
Purification affinity purified
Alternative Name FOXP3 (FOXP3 Antibody Abstract)
Background FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators andis highly conserved across mammals. FOXP3 is essential for normal immunehomeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positiveregulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent inCD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and amore specific marker of regulatory T cells than other T cells. Alternate Names: anti-FOXP3 antibody, anti-JM2 antibody, anti-AIID antibody, anti-IPEX antibody,anti-PIDX antibody, anti-XPID antibody, anti-DIETER antibody, anti-SCURFIN antibody,anti-forkhead box P3 antibody, anti-scurfin antibody, anti-JM2 protein antibody,anti-immunodeficiency antibody, anti-polyendocrinopathy antibody, anti-enteropathyantibody, anti-X-linked antibody, anti-immune dysregulation antibody,anti-polyendocrinopathy antibody, anti-enteropathy antibody, anti-X-linked antibody,anti-Official Symbol antibody, anti-FOXP3 antibody.
Gene Symbol: FOXP3
Gene ID 50943, 20371
UniProt Q9BZS1
Research Area Immunology, Adaptive Immunity, Transcription Factors
Application Notes This FOXP3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. By Western blot, this antibody recognizes bands at ~47.2 kDa representing FOXP3. For IHC-Fr it has been used in mouse thymus from an influenza-infected mouse or thymus from healthy mice (C57BL/6).
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:50, Immunohistochemistry-Frozen 1:50, Western Blot 1:1000-1:5000
Protocol Protocol specific for FOXP3 Antibody Protocol for Foxp3 staining.- Cut 5 mm sections in a cryostat and fix them in cold acetone for 10 minutes- Dry the slides completely at room temperature (30 minutes)- Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 minutes each time)-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin-Incubate slides in a wet chamber at RT for 30 minutes-Dip off the blocking solution from slides and add streptavidin (0.1 mg/ml) for 15 minutes at RT-Wash one time with 0.1 % saponin-Add a similar volume of biotin (0.1 mg/ml) and incubate 15 min at RT- Wash again with 0.1 % saponin- Add rabbit anti-Foxp3 (1:150) and biotin-rat anti-mouse CD4 (1:100) and incubate ON at RT- Next day: Wash slides with PBS and add SA-594 or SA-488 (dissolved in PBS) to detect CD4 T cells (Dilution 1:500 from molecular probes). Incubate 1 h at RT-Wash one time with 0.1 % saponin and add biotin-donkey anti rabbit (Fab, Jackson Immunoresearch). Secondary antibody is diluted in PBS 1:500 and incubated by 3 hrs.- Wash with saponin again and add SA-488 or SA-594 (1:500 in PBS). Incubate 1 h at RT- Wash with PBS 3 times (10 minutes each wash)- Mount slides with prolong gold antifade (Molecular probes)Control: Incubated only with CD4 and no Foxp3Tissue: thymus from an Influenza-infected mouse or thymus from healthy mice (C57BL/6)
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN269718) anti-Forkhead Box P3 (FOXP3) (AA 400-431), (C-Term) antibody
Immunofluorescence (IF) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN269718) Incubated only with CD4 and no Foxp3
Immunofluorescence (IF) image for anti-FOXP3 antibody (Forkhead Box P3) (C-Term) (ABIN269718) Sample: Healthy thymus from a C57BL/6 mouse
Product cited in: Ostroukhova, Qi, Oriss et al.: "Treg-mediated immunosuppression involves activation of the Notch-HES1 axis by membrane-bound TGF-beta." in: The Journal of clinical investigation, Vol. 116, Issue 4, pp. 996-1004, 2006 (PubMed).

Ochando, Yopp, Yang et al.: "Lymph node occupancy is required for the peripheral development of alloantigen-specific Foxp3+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6993-7005, 2005 (PubMed).

Ochs, Ziegler, Torgerson: "FOXP3 acts as a rheostat of the immune response." in: Immunological reviews, Vol. 203, pp. 156-64, 2005 (PubMed).

Polanczyk, Carson, Subramanian et al.: "Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2227-30, 2004 (PubMed).

Catalog No. ABIN269718
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