FOXP3 antibody (Forkhead Box P3) (N-Term)

Details for Product anti-FOXP3 Antibody No. ABIN269719, Supplier: Log in to see
Antigen
  • foxp3
  • FOXP3
  • AIID
  • DIETER
  • IPEX
  • PIDX
  • XPID
  • JM2
  • scurfin
  • sf
  • RGD1562112
  • forkhead box P3
  • forkhead box P3a
  • transcription factor foxp3
  • FOXP3
  • foxp3a
  • LOC100348270
  • foxp3
  • Foxp3
Alternatives
anti-Human FOXP3 antibody for Immunohistochemistry
Epitope
N-Term, AA 1-50
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This FOXP3 antibody is un-conjugated
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Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Supplier
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Immunogen A synthetic peptide made to the N-terminal region of human FOXP3 (between residues 1-50). [UniProt# Q9BZS1]
Purification Immunogen affinity purified
Alternative Name FoxP3 (FOXP3 Antibody Abstract)
Background Gene Symbol: FOXP3
Molecular Weight Theoretical MW: 47.2 kDa
Gene ID 50943
UniProt Q9BZS1
Pathways Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Application Notes Western Blot 1:1000-1:5000, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:150This FOXP3 antibody is useful for Western blot, where a band can be seen at ~47.2 kDa representing FOXP3. This antibody only detects transfected samples. It has not been shown to work on endogenous samples via Western blot. Immunohistochemistry-Paraffin was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for FOXP3 Antibody IHC Protocol for NB600-246Control Tissues: Thymus from healthy (C57BL6/6) and Influenza infected mice.-Cut 5 um sections in a cryostat and fix them in cold acetone for 10 mins.-Dry the slides completely at room temp for 30 mins.-Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 mins each time).-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin.-Incubate slides in a wet chamber at RT for 30 mins.-Dip off the blocking solution from the slides and add streptavidin (0.1mg/ml) for 15 mins at RT.-Wash one time with 0.1 % saponin.-Add similar volume of biotin (0.
1) and incubate 15 min at RT.-Wash one time with 0.1 % saponin.-Add NB600-246 (1:150) ON at RT.-Next day: Wash slides with 0.1 % saponin -Add biotin-donkey anti-rabbit secondary for 3 hrs at RT.- Wash slides with 0.1 % saponin -Add fluorescently labeled streptavidin (Molecular probes) in PBS for 1 hr at RT.-Wash 3x with PBS.-Mount with prolong gold antifade (Molecular probes)IHC Protocol for NB600-246Control Tissues: Thymus from healthy (C57BL6/6) and Influenza infected mice.-Cut 5 um sections in a cryostat and fix them in cold acetone for 10 mins.-Dry the slides completely at room temp for 30 mins.-Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 mins each time).-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin.-Incubate slides in a wet chamber at RT for 30 mins.-Dip off the blocking solution from the slides and add streptavidin (0.1mg/ml) for 15 mins at RT.-Wash one time with 0.1 % saponin.-Add similar volume of biotin (0.
) and incubate 15 min at RT.-Wash one time with 0.1 % saponin.-Add NB600-246 (1:150) ON at RT.-Next day: Wash slides with 0.1 % saponin -Add biotin-donkey anti-rabbit secondary for 3 hrs at RT.- Wash slides with 0.1 % saponin -Add fluorescently labeled streptavidin (Molecular probes) in PBS for 1 hr at RT.-Wash 3x with PBS.-Mount with prolong gold antifade (Molecular probes)
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) (AA 1-50), (N-Term) antibody (ABIN269719) anti-Forkhead Box P3 (FOXP3) (AA 1-50), (N-Term) antibody
Product cited in: Wilcox, Feldman, Wada, Yang, Comfere, Dong, Kwon, Novak, Markovic, Pittelkow, Witzig, Ansell: "B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders." in: Blood, Vol. 114, Issue 10, pp. 2149-58, 2009 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lim, Hillsamer, Banham, Kim: "Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 7, pp. 4180-3, 2005 (PubMed).