Forkhead Box P3 (FOXP3) (N-Term), (AA 1-50) antibody

Details for Product No. ABIN269719
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Antigen
Synonyms foxp3, FOXP3, AIID, DIETER, IPEX, PIDX, XPID, JM2, scurfin, sf, RGD1562112
Epitope
N-Term, AA 1-50
(72), (18), (7), (7), (7), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(195), (124), (47), (15), (15), (14), (12), (7)
Host
Rabbit
(155), (63), (43), (14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(19), (16), (15), (9), (7), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(183), (92), (81), (60), (39), (10), (10), (9), (9), (8), (8), (7), (7), (5), (3), (2)
Pubmed 1 reference available
Quantity 0.025 mL
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Catalog No. ABIN269719
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Immunogen A synthetic peptide made to the N-terminal region of human FOXP3 (between residues 1-50).
Purification affinity purified
Alternative Name FOXP3
Background FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family oftranscriptional regulators and is highly conserved across mammals. FOXP3 is essentialfor normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positiveregulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent inCD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and amore specific marker of regulatory T cells than other T cells. Alternate Names: anti-AIID antibody, anti-DIETER antibody, anti-Forkhead box P3 antibody, anti-foxp3antibody, anti-IPEX antibody, anti-JM2 antibody, anti-PIDX antibody, anti-SCURFINantibody, anti-XPID antibody.
Gene Symbol: FOXP3
Gene ID 50943
UniProt Q9BZS1
Research Area Immunology, Adaptive Immunity, Transcription Factors
Application Notes This FOXP3 antibody is useful for Western blot, where a band can be seen at ~47.2 kDa representing FOXP3. This antibody only detects transfected samples. It has not been shown to work on endogenous samples via Western blot. Immunohistochemistry-Paraffin was reported in scientific literature.
Recommended dilutions: Immunohistochemistry-Paraffin, Western Blot 1:1000-1:5000
Protocol Protocol specific for FOXP3 Antibody IHC Protocol for NB600-246Control Tissues: Thymus from healthy (C57BL6/6) and Influenza infected mice.-Cut 5 um sections in a cryostat and fix them in cold acetone for 10 mins.-Dry the slides completely at room temp for 30 mins.-Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 mins each time).-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin.-Incubate slides in a wet chamber at RT for 30 mins.-Dip off the blocking solution from the slides and add streptavidin (0.1mg/ml) for 15 mins at RT.-Wash one time with 0.1 % saponin.-Add similar volume of biotin (0.
1) and incubate 15 min at RT.-Wash one time with 0.1 % saponin.-Add NB600-246 (1:150) ON at RT.-Next day: Wash slides with 0.1 % saponin -Add biotin-donkey anti-rabbit secondary for 3 hrs at RT.- Wash slides with 0.1 % saponin -Add fluorescently labeled streptavidin (Molecular probes) in PBS for 1 hr at RT.-Wash 3x with PBS.-Mount with prolong gold antifade (Molecular probes)IHC Protocol for NB600-246Control Tissues: Thymus from healthy (C57BL6/6) and Influenza infected mice.-Cut 5 um sections in a cryostat and fix them in cold acetone for 10 mins.-Dry the slides completely at room temp for 30 mins.-Permeabilize tissues by immersing them in 0.1 % saponin, 3 times (5 mins each time).-Block unspecific binding to the slides using 5 % normal donkey serum, 5 % normal rat serum and Fc block (1:500). Everything has to be dissolved in 0.1 % saponin.-Incubate slides in a wet chamber at RT for 30 mins.-Dip off the blocking solution from the slides and add streptavidin (0.1mg/ml) for 15 mins at RT.-Wash one time with 0.1 % saponin.-Add similar volume of biotin (0.
) and incubate 15 min at RT.-Wash one time with 0.1 % saponin.-Add NB600-246 (1:150) ON at RT.-Next day: Wash slides with 0.1 % saponin -Add biotin-donkey anti-rabbit secondary for 3 hrs at RT.- Wash slides with 0.1 % saponin -Add fluorescently labeled streptavidin (Molecular probes) in PBS for 1 hr at RT.-Wash 3x with PBS.-Mount with prolong gold antifade (Molecular probes)
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
General Lim, Hillsamer, Banham et al.: "Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 7, pp. 4180-3, 2005 (PubMed).

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