Notch 2 (NOTCH2) antibody

Antigen: Notch 2 (NOTCH2)

Synonyms: hN2, AGS2, N2, AI853703, NOTCH2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Catalog no.: ABIN269894

Additional Information

Alternative name: NOTCH2

Gene ID: 4853

Immunogen: Synthetic Peptide: C-NAAERTPSHSGHLQaa 2396-2409 (the total protein is 2471 aa).

Cross-Reactivity: Human

Format: Serum

Isotype: IgG

Description: Synthesized in the endoplasmic reticulum as an inactive form which is proteolyticallycleaved by a furin-like convertase in the trans-Golgi network before it reaches theplasma membrane to yield an active, ligand-accessible form. Cleavage results in aC-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding,it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associatedintermediate fragment called notch extracellular truncation (NEXT). This fragment isthen cleaved by presenilin dependent gamma-secretase to release a notch-derivedpeptide containing the intracellular domain (NICD) from the membraneFunctions as areceptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulatecell-fate determination. Upon ligand activation through the released notch intracellulardomain (NICD) it forms a transcriptional activator complex with RBP-J kappa andactivates genes of the enhancer of split locus. Affects the implementation ofdifferentiation, proliferation and apoptotic programs. Alternate Names: anti-N2 antibody.

Specificity: Species Reactivity: Expected to cross-react with Human (100% identity with immunogen) due to sequencehomology. Not yet tested in other species.

Application Details

Application Notes: This antibody has only been tested in ELISA against the peptide immunogen, in which astrong response has been detected. This antibody has not been tested in otherapplications.

Purification: Serum

Buffer: serum with 0.02% sodium azide

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Chen, Oh, Hinman et al.: "Visualization of APP dimerization and APP-Notch2 heterodimerization in living cells using bimolecular fluorescence complementation." in: Journal of neurochemistry, Vol. 97, Issue 1, pp. 30-43, 2006 (PubMed).

Rutenberg, Fischer, Jia et al.: "Developmental patterning of the cardiac atrioventricular canal by Notch and Hairy-related transcription factors." in: Development (Cambridge, England), Vol. 133, Issue 21, pp. 4381-90, 2006 (PubMed).