Notch 2 (NOTCH2) antibody

Details for Product No. ABIN269894
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Antigen
Synonyms AGS2, HJCYS, hN2, AI853703, N2, notch6, sb:cb884
Reactivity
Human
(87), (51), (48), (18), (5), (3), (2), (1), (1)
Host
Rabbit
(83), (9), (4), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(63), (59), (27), (22), (16), (10), (8), (4), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 0.2 mL
Shipping to United States (Change)
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Catalog No. ABIN269894
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Immunogen Synthetic Peptide: C-NAAERTPSHSGHLQaa 2396-2409 (the total protein is 2471 aa).
Isotype IgG
Cross-Reactivity (Details) Expected to cross-react with Human. The peptide sequence shows 100% alignment with human, dog,chimpanzee sequence. Only one (1) amino acid difference is found in mouse,this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat,mouse.
Purification Whole antisera
Alternative Name NOTCH2
Background Synthesized in the endoplasmic reticulum as an inactive form which is proteolyticallycleaved by a furin-like convertase in the trans-Golgi network before it reaches theplasma membrane to yield an active, ligand-accessible form. Cleavage results in aC-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding,it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associatedintermediate fragment called notch extracellular truncation (NEXT). This fragment isthen cleaved by presenilin dependent gamma-secretase to release a notch-derivedpeptide containing the intracellular domain (NICD) from the membraneFunctions as areceptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulatecell-fate determination. Upon ligand activation through the released notch intracellulardomain (NICD) it forms a transcriptional activator complex with RBP-J kappa andactivates genes of the enhancer of split locus. Affects the implementation ofdifferentiation, proliferation and apoptotic programs. Alternate Names: anti-N2 antibody.
Gene Symbol: NOTCH2
Gene ID 4853
Application Notes For western blot experiments, one can expect a band of approximately 110 kDa in size corresponding - active Notch 2 in the appropriate cell lysate.
Recommended dilutions: ELISA 1:30000-1:90000, Immunohistochemistry 1:200-1:800, Immunohistochemistry-Paraffin 1:500, Western Blot 1:400-1:2000
Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate with 0.15 M Sodium Chloride pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Chen, Oh, Hinman et al.: "Visualization of APP dimerization and APP-Notch2 heterodimerization in living cells using bimolecular fluorescence complementation." in: Journal of neurochemistry, Vol. 97, Issue 1, pp. 30-43, 2006 (PubMed).

Rutenberg, Fischer, Jia et al.: "Developmental patterning of the cardiac atrioventricular canal by Notch and Hairy-related transcription factors." in: Development (Cambridge, England), Vol. 133, Issue 21, pp. 4381-90, 2006 (PubMed).

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