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Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (Internal Region) antibody

Details for Product No. ABIN271286, Supplier: Log in to see
Antigen
  • AC017581
  • CG7597
  • Dmel\\CG7597
  • anon-WO0140519.165
  • cg7597
  • dCDK12
  • gi24668141
  • CRK7
  • CRKR
  • CRKRS
  • hCDK12
  • 1810022J16Rik
  • AI646528
  • Crk7
  • Crkrs
  • D11Ertd752e
  • Pksc
  • CG7597 gene product from transcript CG7597-RB
  • Cdc2-related kinase, arginine/serine-rich
  • cyclin-dependent kinase 12
  • Cdk12
  • LOC100354313
  • CDK12
Epitope
Internal Region
18
5
4
4
3
2
1
Reactivity
Dog (Canine), Human, Monkey, Primate
37
5
1
Host
Rabbit
32
5
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
24
20
9
4
4
2
1
1
Options
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Immunogen Synthetic 20 amino acid peptide from internal region of human CDK12/ CRKRS.
Specificity Human CDK12/ CRKRS. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except AKAP12 (55 % ).
Cross-Reactivity (Details) Expected cross reactivity based on sequence identity: Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (100%), Elephant, Bovine, Rabbit (95%), Rat, Bat (90%), Mouse, Hamster, Horse (85%).
Purification Immunogen affinity purified
Alternative Name CrkRS (CRKRS Antibody Abstract)
Background Gene Symbol: CDK12
Gene ID 51755
UniProt Q9NYV4
Research Area Cancer, Tyrosine Kinases
Application Notes Immunohistochemistry-Paraffin 6 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for CrkRS Antibody (NLS7350)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS) (Internal Region) antibody (ABIN271286) Immunohistochemistry-Paraffin: CrkRS Antibody [NLS7350] - Analysis of anti-CDK12 / CR...
Product cited in: Capra, Nuciforo, Confalonieri, Quarto, Bianchi, Nebuloni, Boldorini, Pallotti, Viale, Gishizky, Draetta, Di Fiore: "Frequent alterations in the expression of serine/threonine kinases in human cancers." in: Cancer research, Vol. 66, Issue 16, pp. 8147-54, 2006 (PubMed).