SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Ser423), (Ser425) antibody

Details for Product No. ABIN271426
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Ser423, Ser425
(55), (46), (40), (33), (32), (27), (23), (14), (13), (12), (12), (10), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Mouse (Murine)
(407), (193), (178), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(385), (37)
(18), (17), (17), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (6), (1)
Western Blotting (WB)
(298), (196), (186), (85), (70), (60), (30), (22), (19), (17), (16), (16), (14), (10), (3), (2), (2), (1)
Pubmed 8 references available
Quantity 0.1 mL
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Catalog No. ABIN271426
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Immunogen The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Smad3 that contains serine 423 and serine 425.
Isotype IgG
Specificity SMAD3 [Ser423/Ser425]
Cross-Reactivity (Details) Human Smad3 (100% homologous) has not been tested, but is expected to react. This antibody cross-reacts with Smad2 in cell systems expressing a high level of dually phosphorylated Smad2 (58 kDa).
Purification affinity purified
Alternative Name SMAD3
Background Smad3 is a ~50 kDa member of a family of proteins that act as key mediators of TGF-bsuperfamily signaling in cell proliferation, differentiation and development. The Smadfamily is divided into three subclasses: receptor-regulated Smads, activin/TGF-breceptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), thecommon partner, (Smad4) that functions via its interaction to the various Smads, andthe inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 uponTGF-b stimulation and translocates as a complex into the nucleus, allowing its binding toDNA and transcription factors. Phosphorylation of the two TGF-b-dependent serines 423and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity andTGF-b signaling. Alternate Names: anti-HSPC193 antibody, anti-HST17436 antibody, anti-JV152 antibody, anti-MAD(mothers against decapentaplegic Drosophila) homolog 3 antibody, anti-MAD3 antibody,anti-MADH3 antibody, anti-Mothers against DPP homolog 3 antibody, anti-SMA andMAD related protein 3 antibody, anti-SMAD3 antibody, anti-DKFZP586N0721 antibody,anti-DKFZp686J10186 antibody, anti-antibody.
Gene Symbol: SMAD3
Gene ID 4088
Research Area Signaling, Transcription Factors
Application Notes This antibody is suitable for use in Western blotting. *The optimal concentration should be determined for each specific application.
Recommended dilutions: Western Blot 1/1000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS pH 7.3, 50 % glycerol, with 1.0mg/ml BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Yoshikawa, Hishikawa, Marumo et al.: "Inhibition of histone deacetylase activity suppresses epithelial-to-mesenchymal transition induced by TGF-beta1 in human renal epithelial cells." in: Journal of the American Society of Nephrology : JASN, Vol. 18, Issue 1, pp. 58-65, 2007 (PubMed).

Runyan, Schnaper, Poncelet: "The phosphatidylinositol 3-kinase/Akt pathway enhances Smad3-stimulated mesangial cell collagen I expression in response to transforming growth factor-beta1." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2632-9, 2004 (PubMed).

Furukawa, Matsuzaki, Mori et al.: "p38 MAPK mediates fibrogenic signal through Smad3 phosphorylation in rat myofibroblasts." in: Hepatology (Baltimore, Md.), Vol. 38, Issue 4, pp. 879-89, 2003 (PubMed).

Felici, Wurthner, Parks et al.: "TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling." in: The EMBO journal, Vol. 22, Issue 17, pp. 4465-77, 2003 (PubMed).

Hayashida, Decaestecker, Schnaper: "Cross-talk between ERK MAP kinase and Smad signaling pathways enhances TGF-beta-dependent responses in human mesangial cells." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 11, pp. 1576-8, 2003 (PubMed).

Roberts, Russo, Felici et al.: "Smad3: a key player in pathogenetic mechanisms dependent on TGF-beta." in: Annals of the New York Academy of Sciences, Vol. 995, pp. 1-10, 2003 (PubMed).

Runyan, Schnaper, Poncelet: "Smad3 and PKCdelta mediate TGF-beta1-induced collagen I expression in human mesangial cells." in: American journal of physiology. Renal physiology, Vol. 285, Issue 3, pp. F413-22, 2003 (PubMed).

Piek, Moustakas, Kurisaki et al.: "TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 24), pp. 4557-68, 2000 (PubMed).

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