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SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody

Antigen

SMAD, Mothers Against DPP Homolog 3 (SMAD3)
Synonyms MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZp586N0721, DKFZp686J10186
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
8 references available
Catalog no. ABIN271426
Quantity 0.1 ml  (1 mg/ml)  (Variants)
Price Product not available in this region.
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Additional Information
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Alternative name SMAD3
Immunogen The antiserum was produced against a chemically synthesized phosphopeptide derivedfrom a region of human Smad3 that contains serine 423 and serine 425.
Cross-Reactivity Mouse (Murine)
Isotype IgG
Description Smad3 is a ~50 kDa member of a family of proteins that act as key mediators of TGF-bsuperfamily signaling in cell proliferation, differentiation and development. The Smadfamily is divided into three subclasses: receptor-regulated Smads, activin/TGF-breceptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), thecommon partner, (Smad4) that functions via its interaction to the various Smads, andthe inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 uponTGF-b stimulation and translocates as a complex into the nucleus, allowing its binding toDNA and transcription factors. Phosphorylation of the two TGF-b-dependent serines 423and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity andTGF-b signaling. Alternate Names: anti-HSPC193 antibody, anti-HST17436 antibody, anti-JV152 antibody, anti-MAD(mothers against decapentaplegic Drosophila) homolog 3 antibody, anti-MAD3 antibody,anti-MADH3 antibody, anti-Mothers against DPP homolog 3 antibody, anti-SMA andMAD related protein 3 antibody, anti-SMAD3 antibody, anti-DKFZP586N0721 antibody,anti-DKFZp686J10186 antibody, anti-antibody.
Specificity Species Reactivity: Mouse Smad3. Human Smad3 (100% homologous) has not been tested, but isexpected to react. This antibody does not cross-react with Smad2.

Application Details
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Application Notes Suggested working dilutions: Western Blot 1/1000. Positive Controls: NMuMG cells +/- TGF-b.
Concentration 1 mg/ml
Purification Aff - Purified
Buffer PBS. Preservative: 0.05% Sodium Azide.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Research Area Signaling, Transcription Factors
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Piek, Moustakas, Kurisaki et al.: "TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 24), pp. 4557-68, 2000 (PubMed).

Runyan, Schnaper, Poncelet: "Smad3 and PKCdelta mediate TGF-beta1-induced collagen I expression in human mesangial cells." in: American journal of physiology. Renal physiology, Vol. 285, Issue 3, pp. F413-22, 2003 (PubMed).

Roberts, Russo, Felici et al.: "Smad3: a key player in pathogenetic mechanisms dependent on TGF-beta." in: Annals of the New York Academy of Sciences, Vol. 995, pp. 1-10, 2003 (PubMed).

Hayashida, Decaestecker, Schnaper: "Cross-talk between ERK MAP kinase and Smad signaling pathways enhances TGF-beta-dependent responses in human mesangial cells." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 11, pp. 1576-8, 2003 (PubMed).

Felici, Wurthner, Parks et al.: "TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling." in: The EMBO journal, Vol. 22, Issue 17, pp. 4465-77, 2003 (PubMed).

Furukawa, Matsuzaki, Mori et al.: "p38 MAPK mediates fibrogenic signal through Smad3 phosphorylation in rat myofibroblasts." in: Hepatology (Baltimore, Md.), Vol. 38, Issue 4, pp. 879-89, 2003 (PubMed).

Runyan, Schnaper, Poncelet: "The phosphatidylinositol 3-kinase/Akt pathway enhances Smad3-stimulated mesangial cell collagen I expression in response to transforming growth factor-beta1." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2632-9, 2004 (PubMed).

Yoshikawa, Hishikawa, Marumo et al.: "Inhibition of histone deacetylase activity suppresses epithelial-to-mesenchymal transition induced by TGF-beta1 in human renal epithelial cells." in: Journal of the American Society of Nephrology : JASN, Vol. 18, Issue 1, pp. 58-65, 2007 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "SMAD, Mothers Against DPP Homolog 3 (SMAD3)", type "Antibodies"
Hosts Rabbit (154), Mouse (9)
Reactivities Human (154), Mouse (Murine) (72), Rat (Rattus) (72), Cow (Bovine) (39), Chicken (30), Horse (Equine) (20), Pig (Porcine) (12), Sheep (Ovine) (10), Dog (Canine) (6), Xenopus laevis (2), Zebrafish (2), Xenopus tropicalis (1)
Applications Western Blotting (WB) (93), ELISA (73), Immunofluorescence (IF) (53), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Immunoprecipitation (IP) (7), Fluorescence Microscopy (FM) (4), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes Internal Region (13), pSer425 (13), pSer208 (8), pSer213 (8), pSer204 (6), C-Term (4), pSer425,pSer423 (4), AA 100-150 (3), Center (3), Ser425 (3), AA 206-220 (2), AA 417-425 (2), N-Term (2), Ser208 (2), pSer423, pSer425 (2), pSer423,pSer425 (2), pThr179 (2), pThr179,Internal Region (2), AA 122-365 (1), AA 417-425,pSer425,pSer423 (1), Center,Asn206 (1), Center,Asp201 (1), Ser213 (1), Ser423, Ser425 (1), pSer100 (1), pSer423,pSer425,Internal Region (1), pSer695 (1), pThr115 (1), pThr160 (1), pThr91 (1)