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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
|Synonyms||MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZp586N0721, DKFZp686J10186|
Alternatives Western Blotting (WB)
|8 references available|
|Quantity||0.1 ml (1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derivedfrom a region of human Smad3 that contains serine 423 and serine 425.|
|Description||Smad3 is a ~50 kDa member of a family of proteins that act as key mediators of TGF-bsuperfamily signaling in cell proliferation, differentiation and development. The Smadfamily is divided into three subclasses: receptor-regulated Smads, activin/TGF-breceptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), thecommon partner, (Smad4) that functions via its interaction to the various Smads, andthe inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 uponTGF-b stimulation and translocates as a complex into the nucleus, allowing its binding toDNA and transcription factors. Phosphorylation of the two TGF-b-dependent serines 423and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity andTGF-b signaling. Alternate Names: anti-HSPC193 antibody, anti-HST17436 antibody, anti-JV152 antibody, anti-MAD(mothers against decapentaplegic Drosophila) homolog 3 antibody, anti-MAD3 antibody,anti-MADH3 antibody, anti-Mothers against DPP homolog 3 antibody, anti-SMA andMAD related protein 3 antibody, anti-SMAD3 antibody, anti-DKFZP586N0721 antibody,anti-DKFZp686J10186 antibody, anti-antibody.|
|Specificity||Species Reactivity: Mouse Smad3. Human Smad3 (100% homologous) has not been tested, but isexpected to react. This antibody does not cross-react with Smad2.|
|Application Notes||Suggested working dilutions: Western Blot 1/1000. Positive Controls: NMuMG cells +/- TGF-b.|
|Purification||Aff - Purified|
|Buffer||PBS. Preservative: 0.05% Sodium Azide.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Research Area||Signaling, Transcription Factors|
|Restrictions||For Research Use only|
Piek, Moustakas, Kurisaki et al.: "TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells." in: Journal of cell science, Vol. 112 ( Pt 24), pp. 4557-68, 2000 (PubMed).
Runyan, Schnaper, Poncelet: "Smad3 and PKCdelta mediate TGF-beta1-induced collagen I expression in human mesangial cells." in: American journal of physiology. Renal physiology, Vol. 285, Issue 3, pp. F413-22, 2003 (PubMed).
Roberts, Russo, Felici et al.: "Smad3: a key player in pathogenetic mechanisms dependent on TGF-beta." in: Annals of the New York Academy of Sciences, Vol. 995, pp. 1-10, 2003 (PubMed).
Hayashida, Decaestecker, Schnaper: "Cross-talk between ERK MAP kinase and Smad signaling pathways enhances TGF-beta-dependent responses in human mesangial cells." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 11, pp. 1576-8, 2003 (PubMed).
Felici, Wurthner, Parks et al.: "TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling." in: The EMBO journal, Vol. 22, Issue 17, pp. 4465-77, 2003 (PubMed).
Furukawa, Matsuzaki, Mori et al.: "p38 MAPK mediates fibrogenic signal through Smad3 phosphorylation in rat myofibroblasts." in: Hepatology (Baltimore, Md.), Vol. 38, Issue 4, pp. 879-89, 2003 (PubMed).
Runyan, Schnaper, Poncelet: "The phosphatidylinositol 3-kinase/Akt pathway enhances Smad3-stimulated mesangial cell collagen I expression in response to transforming growth factor-beta1." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2632-9, 2004 (PubMed).
Yoshikawa, Hishikawa, Marumo et al.: "Inhibition of histone deacetylase activity suppresses epithelial-to-mesenchymal transition induced by TGF-beta1 in human renal epithelial cells." in: Journal of the American Society of Nephrology : JASN, Vol. 18, Issue 1, pp. 58-65, 2007 (PubMed).