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Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody

Antigen

Poly (ADP-Ribose) Polymerase 1 (PARP1)
Synonyms PARP, PPOL, ADPRT, ADPRT1, PARP-1, pADPRT-1, RT6, ART2, CD296, MGC133217, Yac-1, Adprt, Parp-1, MGC93658, ART1, ART7.2, ART7.1
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunofluorescence (IF), Western Blotting (WB)
4 references available
Catalog no. ABIN271522
Quantity 0.1 ml  (Variants)
Price Product not available in this region.
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Additional Information
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Alternative name PARP1
Gene ID 142
Immunogen Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) ofhuman PARP.
Cross-Reactivity Human
Isotype IgG
Description Alternate Names: anti-ADP ribosyltransferase NAD+ antibody, anti-pADPRT1 antibody, anti-PARP1antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-PolyADP ribose polymerase 1 antibody, anti-Poly ADP ribose synthetase 1 antibody,anti-PPOL antibody.
Specificity This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can beused as marker for detecting apoptotic cells. Species Reactivity: Cross-reacts with Cow and Human. Not yet tested in other species. Localization: Nuclear.

Application Details
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Application Notes Positive Controls: HeLa cells treated with staurosporine at 0.5 uM for 5 hours or bovine PARP cleaved bycaspase 3.
Purification Aff - Purified
Buffer Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG,protease free). pH. Preservative: 0.05% Sodium AzideConstituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG,protease free). pH.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Duriez, Shah: "Cleavage of poly(ADP-ribose) polymerase: a sensitive parameter to study cell death." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 75, Issue 4, pp. 337-49, 1998 (PubMed).

Germain, Affar, DAmours et al.: "Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28379-84, 1999 (PubMed).

Leemans, Juffermans, Florquin et al.: "Depletion of alveolar macrophages exerts protective effects in pulmonary tuberculosis in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4604-11, 2001 (PubMed).

Turturro, Arnold, Frist et al.: "Model of inhibition of the NPM-ALK kinase activity by herbimycin A." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 8, Issue 1, pp. 240-5, 2002 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Poly (ADP-Ribose) Polymerase 1 (PARP1)", type "Antibodies"
Hosts Rabbit (91), Mouse (22)
Reactivities Human (89), Mouse (Murine) (22), Rat (Rattus) (20), Cow (Bovine) (12), Chicken (2), Dog (Canine) (2), Fruit Fly (Drosophila melanogaster) (2), Hamster (2), Monkey (2), Xenopus laevis (2), All Species (1), Cat (Feline) (1)
Applications Western Blotting (WB) (98), ELISA (52), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunocytochemistry (ICC) (24), Immunoprecipitation (IP) (18), Immunohistochemistry (IHC) (15), Immunofluorescence (IF) (11), Dot Blot (DB) (9), Flow Cytometry (FACS) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Functional Studies (Func) (1), Neutralization (Neut) (1)
Conjugates FITC (1)
Epitopes N-Term (17), C-Term (8), cleaved (5), AA 215-525 (2), AA 396-412 (2), Internal Region (2), AA 1-1014 (1), AA 1-17 (1), AA 197-214 (1), AA 214-225 (1), AA 215-228 (1), Gly623 (1), Internal Region,AA 155-185 (1), Internal Region,AA 251-281 (1), Internal Region,AA 353-382 (1), Internal Region,AA 358-387 (1), Internal Region,AA 590-618 (1), Internal Region,AA 759-789 (1), N-Term,AA 123-151 (1), N-Term,AA 26-56 (1), N-Term,AA 57-87 (1), pSer177 (1), pSer274 (1), pSer372 (1), pSer373 (1), pSer41 (1), pSer782 (1), pSer864 (1), pThr368 (1), pThr373 (1)