Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1)

Synonyms: VR1, DKFZp434K0220, Vr1, Vr1l1, VR.5'sv, VR-1, OTRPC1, TRPV1beta, TRPV1alpha, TRPV1, Trpv1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN271603

Additional Information

Alternative name: TRPV1 / Vanilloid receptor 1

Immunogen: A synthetic peptide (C-YFSHLKEYVAS) as part of mouse capsaicin receptor protein(aa: 531-541) conjugated to KLH.

Description: The cloned VR1 is about 95-kDa protein. Both N- and C- termini of VR1 are intracellular.A hexa-transmembrane domains with an additional intra-membrane loop connecting thefifth and sixth transmembrane has been ascribed to the structure of this molecule. Thestructure and amino acid sequence of VR1 is similar to those of the transient receptorpotential (TRP) family of cation channels. FUNCTION: Receptor-activated non-selectivecalcium permeant cation channel involved in detection of noxious chemical and thermalstimuli. Seems to mediate proton influx and may be involved in intracellular acidosis innociceptive neurons. May be involved in mediation of inflammatory pain andhyperalgesia. Sensitized by a phosphatidylinositol second messenger system activatedby receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation byvanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits atime- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractorystate. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heatand vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can beactivated by endogenous compounds, including 12-hydroperoxytetraenoic acid, andendocannabinoids, like anandamide, and bradykinin. SUBUNIT: Self-associates.Probably homotetramer. May also form a heteromeric channel with TRPV3. Interactswith calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably byforming a trimeric complex. SUBCELLULAR LOCATION: Cell membrane, multi-passmembrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced byalternative splicing. TISSUE SPECIFICITY: Predominantly expressed in trigeminal anddorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain andperipheral blood mononuclear cells. Alternate Names: anti-VR1 antibody, anti-Transient receptor potential cation channel subfamily V member1 antibody, anti-TrpV1 antibody, anti-osm-9-like TRP channel 1 antibody, anti-OTRPC1antibody, anti-Vanilloid receptor 1 antibody, anti-Vanilloid receptor type 1-like antibody. Related Diseases: TRP channels

Specificity: Immunohistochemical analysis of rat DRG indicates a superb degree of specificity forthis serum. Species Reactivity: This antibody is known to react with mouse and rat VR1. Localization: Accession Number: TRPV1_MOUSE.

Application Details

Application Notes: IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in otherapplications. The optimal dilution should be determined by the end user.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Research Area: Receptors, Signaling

Restrictions: For Research Use only

Publications

Tominaga, Caterina, Malmberg et al.: "The cloned capsaicin receptor integrates multiple pain-producing stimuli." in: Neuron, Vol. 21, Issue 3, pp. 531-43, 1998 (PubMed).

Nagy, Suantha, Jancsuo et al.: "The role of the vanilloid (capsaicin) receptor (TRPV1) in physiology and pathology." in: European journal of pharmacology, Vol. 500, Issue 1-3, pp. 351-69, 2004 (PubMed).