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Amyloid beta (A4) Precursor Protein (APP) antibody

Antigen

Amyloid beta (A4) Precursor Protein (APP)
Synonyms APP, APP-like, APPL, Abeta, BcDNA:GH04413, EG:65F1.5, DmelCG7727, CG7727, AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, CTFgamma, aaa, ad1, pn2, abpp, appi, cvap, abeta, MGC88882, ctfgamma
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro))
3 references available
Catalog no. ABIN271611
Quantity 0.1 ml  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Amyloid beta A4 protein / APP
Immunogen Synthetic peptides (C-ETHLHW HTVAKET, aa: 145-157, C-HAH FQKAKERLEAKHRER, aa: 388-405, C-KKKQYTS IHHGVVE, aa: 724-737) as parts of human APPisoform A conjugated to KLH.
Cross-Reactivity Rat (Rattus)
Description FUNCTION: Functions as a cell surface receptor and performs physiological functionson the surface of neurons relevant to neurite growth, neuronal adhesion andaxonogenesis. Involved in cell mobility and transcription regulation throughprotein-protein interactions. Can promote transcription activation through binding toAPBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples toapoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o)alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonaltransport of beta-secretase and presenilin 1. May be involved in copperhomeostasis/oxidative stress through copper ion reduction. Can regulate neuriteoutgrowth through binding to components of the extracellular matrix such as heparinand collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelatorswith metal-reducing activity. Bind transient metals such as copper, zinc and iron. Ratand mouse beta-amyloid peptides bind only weakly transient metals and have littlereducing activity due to substitutions of transient metal chelating residues. Beta-APP42may activate mononuclear phagocytes in the brain and elicit inflammatory responses.Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity).FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides,including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via itsC-terminus, to the PID domain of several cytoplasmic proteins, including APBB familymembers, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1,APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, itbinds MAPT via the MT-binding domains. Associates with microtubules in the presenceof ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, withGNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloidassociates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues:kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In thecerebellum, all the isoforms are abundantly expressed in Purkinje cells. Alternate Names: anti-AAA antibody, anti-ABETA antibody, anti-ABPP antibody, anti-AD1 antibody,anti-APPI antibody, anti-CTFgamma antibody, anti-CVAP antibody, anti-PN2 antibody. Related Diseases: Other Neuronal
Specificity Specificity for APP was confirmed by IHC. Species Reactivity: Reacts with rat. Localization: Accession Number: APP_HUMAN.

Application Details
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Application Notes Suggested working dilutions: immunohistochemistry 1:500 to 1:3000
Buffer Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.
Storage Store at 4C short term. Aliquot and store at -20 long term. Avoid freeze-thaw cycles.
Research Area Neurology, Alzheimer's Disease
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Wilson, Doms, Lee: "Distinct presenilin-dependent and presenilin-independent gamma-secretases are responsible for total cellular Abeta production." in: Journal of neuroscience research, Vol. 74, Issue 3, pp. 361-9, 2003 (PubMed).

Andreasen, Sjoegren, Blennow: "CSF markers for Alzheimer's disease: total tau, phospho-tau and Abeta42." in: The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry, Vol. 4, Issue 4, pp. 147-55, 2003 (PubMed).

Guuenette: "Mechanisms of Abeta clearance and catabolism." in: Neuromolecular medicine, Vol. 4, Issue 3, pp. 147-60, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Amyloid beta (A4) Precursor Protein (APP)", type "Antibodies"
Hosts Rabbit (234), Mouse (67), Goat (34), Chicken (3)
Reactivities Human (299), Mouse (Murine) (146), Rat (Rattus) (134), Cow (Bovine) (75), Dog (Canine) (70), Chicken (69), Pig (Porcine) (68), Horse (Equine) (56), Monkey (9), Sheep (Ovine) (5), Rabbit (3), Guinea Pig (2), Xenopus laevis (2), Zebrafish (2), Cat (Feline) (1), Fruit Fly (Drosophila melanogaster) (1), Primate (1)
Applications Western Blotting (WB) (239), ELISA (123), Immunofluorescence (IF) (84), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (75), Immunohistochemistry (IHC) (62), Immunoprecipitation (IP) (23), Flow Cytometry (FACS) (12), Immunohistochemistry (Fixed) (IHC (fx)) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Enzyme Immunoassay (EIA) (1)
Conjugates Alexa Fluor 350 (8), Alexa Fluor 488 (8), Alexa Fluor 555 (8), Alexa Fluor 647 (8), PE,Cy5.5 (8), Biotin (4), FITC (4), HRP (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes N-Term (26), C-Term (21), Transcript Variant 1 (10), pThr668 (10), pThr743 (9), pSer198 (6), pSer730 (6), pTyr757 (5), AA 44-63 (4), AA 737-751 (4), AA 292-312 (3), Internal Region (3), Thr668 (3), AA 18-289 (2), AA 653-662 (2), AA 660-669 (2), AA 681-695 (2), AA 747-761 (2), Frameshift Mutant (2), pTyr756 (2), AA 1-14 (1), AA 1-16 (1), AA 339-348 (1), AA 350-450 (1), AA 36-42 (1), AA 671-685 (1), AA 73-7 (1), C-Term,Asp672 (1), Center (1), Extracellular Domain (1), Frameshift Mutant, AA 339-348 (1), Frameshift Mutant, C-Term (1), KPI Domain (1), N-Term,AA 18-32 (1), OX-2 Domain (1), Precursor, AA 653-662 (1), Precursor, C-Term, AA 737-751 (1), Ser730 (1), Thr743, Thr668 (1), Tyr756 (1), Wild Type (1), pSer129 (1), pSer182 (1), pSer264 (1), pSer347 (1), pSer498,pSer490 (1), pThr743, pThr668 (1)