Amyloid beta (A4) Precursor Protein (APP) antibody

Details for Product No. ABIN271612
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Antigen
Reactivity
Rat (Rattus)
(693), (352), (288), (212), (202), (197), (191), (71), (13), (12), (4), (2), (2), (1), (1)
Host
Rabbit
(495), (198), (41), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(29), (26), (24), (17), (17), (17), (17), (17), (17), (17), (17), (9), (5), (5), (4), (4), (4), (4), (2), (2)
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(471), (245), (185), (159), (156), (61), (48), (42), (26), (23), (7), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN271612
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Immunogen A synthetic peptide (HMNVQNGKWESDPSGTKTC, aa: 44-62) as part of mouse APP isoform A conjugated to the immunogenic protein Blue Carrier Protein
Specificity Specificity for APP was confirmed by IHC.
Cross-Reactivity (Details) Reactivity with other species has not yet been tested.
Purification Whole antisera
Alternative Name Amyloid beta A4 protein / APP
Background FUNCTION: Functions as a cell surface receptor and performs physiological functionson the surface of neurons relevant to neurite growth, neuronal adhesion andaxonogenesis. Involved in cell mobility and transcription regulation throughprotein-protein interactions. Can promote transcription activation through binding toAPBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples toapoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o)alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonaltransport of beta-secretase and presenilin 1. May be involved in copperhomeostasis/oxidative stress through copper ion reduction. Can regulate neuriteoutgrowth through binding to components of the extracellular matrix such as heparinand collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelatorswith metal-reducing activity. Bind transient metals such as copper, zinc and iron. Ratand mouse beta-amyloid peptides bind only weakly transient metals and have littlereducing activity due to substitutions of transient metal chelating residues. Beta-APP42may activate mononuclear phagocytes in the brain and elicit inflammatory responses.Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity).FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides,including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via itsC-terminus, to the PID domain of several cytoplasmic proteins, including APBB familymembers, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1,APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, itbinds MAPT via the MT-binding domains. Associates with microtubules in the presenceof ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, withGNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloidassociates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues:kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In thecerebellum, all the isoforms are abundantly expressed in Purkinje cells. Alternate Names: anti-AAA antibody, anti-ABETA antibody, anti-ABPP antibody, anti-AD1 antibody,anti-APPI antibody, anti-CTFgamma antibody, anti-CVAP antibody, anti-PN2 antibody. Related Diseases: Other Neuronal
Gene Symbol: APP
Gene ID 351
Research Area Neurology, Alzheimer's Disease
Application Notes Immunohistochemistry.Recommended to be used at a dilution of 1:500 to 1:3000. This antiserum has not yet been tested for western blot. The optimal working dilution should be determined by the end user.
Recommended dilutions: Immunohistochemistry-Paraffin 1:500-1:3000, Immunohistochemistry
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Wilson, Doms, Lee: "Distinct presenilin-dependent and presenilin-independent gamma-secretases are responsible for total cellular Abeta production." in: Journal of neuroscience research, Vol. 74, Issue 3, pp. 361-9, 2003 (PubMed).

Andreasen, Sjögren, Blennow: "CSF markers for Alzheimer's disease: total tau, phospho-tau and Abeta42." in: The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry, Vol. 4, Issue 4, pp. 147-55, 2003 (PubMed).

Guénette: "Mechanisms of Abeta clearance and catabolism." in: Neuromolecular medicine, Vol. 4, Issue 3, pp. 147-60, 2004 (PubMed).

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