Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2)

Synonyms: VRL, VRL1, VRL-1, MGC12549, GRC, Vrl1, OTRPC2, MGC105451, TRPV2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN271617

Additional Information

Alternative name: TRPV2 / Vanilloid receptor-like protein 1

Gene ID: 51393, 29465

Immunogen: A synthetic peptide (C-KNSASEE DHLPLQVLQSP) as part of rat VRL-1 protein (aa:744-761) conjugated to KLH.

Isotype: IgG

Description: Caterina et al has identified a structurally related receptor, VRL-1, that does not respondto capsaicin, acid or moderate heat. Instead, VRL-1 is activated by high temperatures,with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is mostprominently expressed by a subset of medium- to large-diameter neurons, making it acandidate receptor for transducing high-threshold heat responses in this class of cells.VRL-1 transcripts are not restricted to the sensory nervous system, indicating that thischannel may be activated by stimuli other than heat. VRL-1 is one of the six members ofthe vanilloid-like TRP-channel family which is now known as TRPV family. FUNCTION:Calcium-permeable, non-selective cation channel with an outward rectification. Seemsto be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogeneticneuropeptide/head activator. May transduce physical stimuli in mast cells. Activated bytemperatures higher than 52 degrees Celsius, is not activated by vanilloids and acidicpH. SUBUNIT: Homotetramer (Probable). Interacts with a cAMP-dependent proteinkinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts withRGA. SUBCELLULAR LOCATION: Cell membrane, multi-pass membrane protein.Cytoplasm. Translocates from the cytoplasm to the plasma membrane upon ligandstimulation. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal rootganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsalcolumns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA.SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY:Contains 3 ANK repeats. Alternate Names: anti-Transient receptor potential cation channel subfamily V member 2 antibody,anti-TrpV2 antibody, anti-osm-9-like TRP channel 2 antibody, anti-OTRPC2 antibody,anti-Vanilloid receptor-like protein 1 antibody, anti-Stretch-activated channel 2B antibody. Related Diseases: TRP channels

Specificity: Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a highlevel of specificity for this antiserum. Specificity was also shown by Western blot. Species Reactivity: Reacts with rat and human. Localization: Accession Number: TRPV2_RAT.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 0.5-1 ug,immunofluorescence 0.5-1 ug,Western Blot 0.5-1 ug,Immunohistochemistry-Paraffin 0.5-1 ug

Buffer: Reconstitute in 50 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Caterina, Rosen, Tominaga et al.: "A capsaicin-receptor homologue with a high threshold for noxious heat." in: Nature, Vol. 398, Issue 6726, pp. 436-41, 1999 (PubMed).

Jahnel, Bender, Muenter et al.: "Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression." in: European journal of biochemistry / FEBS, Vol. 270, Issue 21, pp. 4264-71, 2003 (PubMed).