Huntingtin Associated Protein 1 (HAP1) antibody

Antigen: Huntingtin Associated Protein 1 (HAP1)

Synonyms: HLP, HAP2, HIP5, hHLP1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN271628

Additional Information

Alternative name: HAP1

Immunogen: Recombinant rat HAP-1.

Cross-Reactivity: Rat (Rattus)

Description: Huntington',s disease (HD), a neurodegenerative disorder characterized by loss ofstriatal neurons, is caused by an expansion of a polyglutamine tract in the HD proteinhuntingtin. It is a progressive disorder that generally begins in middle age withabnormalities of movement, cognition, personality and mood. Huntingtin-associatedprotein-1 is highly expressed in brain and shown to mediate the neuropathology of HD.The human protein interacts with huntingtin, with two cytoskeletal proteins (dynactin andpericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulatedtyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinasesubstrate suggest a role for this protein in vesicular trafficking or organelle transport.Two transcripts encoding different isoforms have been identified but one is anonsense-mediated decay (NMD) candidate. Alternate Names: anti-HAP 1 antibody, anti-HAP 2 antibody, anti-HAP1 antibody, anti-HAP2 antibody,anti-HAPP antibody, anti-hHAP1 antibody, anti-hHLP1 antibody, anti-HIP 5 antibody,anti-HIP5 antibody, anti-HLP antibody, anti-HLP1 antibody, anti-Neuroan 1 antibody,anti-Neuroan1 antibody. Related Diseases: Other Neuronal

Specificity: Specificity for HAP-1 was confirmed by IHC. Species Reactivity: Reacts with rat. Localization: Accession Number: HAP1_RAT.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1:500 to 1:3000

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Storage: Store at 4C short term. Aliquot and store at -20 long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Tang, Tu, Chan et al.: "Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1." in: Neuron, Vol. 39, Issue 2, pp. 227-39, 2003 (PubMed).

Zhou, Cao, Wang et al.: "Huntingtin forms toxic NH2-terminal fragment complexes that are promoted by the age-dependent decrease in proteasome activity." in: The Journal of cell biology, Vol. 163, Issue 1, pp. 109-18, 2003 (PubMed).

Qin, Wang, Sapp et al.: "Huntingtin bodies sequester vesicle-associated proteins by a polyproline-dependent interaction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 1, pp. 269-81, 2004 (PubMed).

Harper, Staber, He et al.: "RNA interference improves motor and neuropathological abnormalities in a Huntington's disease mouse model." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 16, pp. 5820-5, 2005 (PubMed).

Pardo, Colin, Ruegulier et al.: "Inhibition of calcineurin by FK506 protects against polyglutamine-huntingtin toxicity through an increase of huntingtin phosphorylation at S421." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 5, pp. 1635-45, 2006 (PubMed).