Contactin 6 (CNTN6) antibody

Details for Product No. ABIN271641
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Antigen
Synonyms NB3, NB-3, DKFZp459A015
Reactivity
Human
(25), (25), (14), (13), (12), (12)
Host
Rabbit
(28), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro))
(17), (12), (10), (6), (4), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN271641
Quantity 0.1 mL
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Immunogen A synthetic peptide (GYPSPHYRWKQ) as part of mouse contactin-6 conjugated to KLH.
Specificity This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulationof neurons and in the trigeminal nerve root, where staining is restricted to theoligodendrocytes. Species Reactivity: Confirmed to react with rat Contactin-6 and expected to recognise human and mouse. Localization: Accession Number: CNTN6_MOUSE.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Alternative Name Contactin-6
Background The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3subgroup member of immunoglobulin superfamily. It is expressed exclusively in thenervous system and mainly upregulated at the early postnatal stage during mouse braindevelopment. Employing Northern blot analysis Kamei et al found that amongst differentregions of the adult human nervous system cerebellum expressed highest level of NB-3mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast,the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 likeother neural recognition molecules plays a vitally important role in axonal guidanceduring development, plasticity, and maintenance of synaptic connections in the adultbrain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate inoligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of theNotch intracellular domain and promotes oligodendrogliogenesis from progenitor cellsand differentiation of oligodendrocyte precursor cells via Deltex1. In primaryoligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence,the NB-3/Notch signaling pathway may be worthwhile a closer examination for itspotential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-386% identity in nucleotide sequences and 90% identity in amino acid sequences.FUNCTION: Contactins mediate cell surface interactions during nervous systemdevelopment. Participates in oligodendrocytes generation by acting as a ligand ofNOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through thereleased notch intracellular domain (NICD) and subsequent translocation to the nucleus.Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane,lipid-anchor, GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced byalternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it ispreferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebralcortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons andmesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum.DEVELOPMENTAL STAGE: Highly expressed after birth, reaching a maximum at thepostnatal day 7, and declines thereafter in the cerebrum, whereas it increases in thecerebellum to adulthood. MISCELLANEOUS: Mice lacking CNTN6 are viable and fertile,the formation and organization of all nuclei and layers throughout the brains areapparently normal. They are however slow to learn to stay on the rotating rod in therotorod test during repeated trials, and display dysfunction of equilibrium and vestibularsenses in the wire hang and horizontal rod-walking tests. SIMILARITY: Belongs to theimmunoglobulin superfamily. Contactin family. SIMILARITY: Contains 4 fibronectintype-III domains. SIMILARITY: Contains 6 Ig-like C2-type (immunoglobulin-like)domains. Alternate Names: anti-Neural recognition molecule NB-3 antibody, anti-mNB-3 antibody, biosensis. Related Diseases: Adhesion Molecules
Gene ID 27255, 53870, 27256
Application Notes IHC. A dilution of 1:1000 to 1: 4000 is recommended. The optimal working dilutionshould be determined by the end user.
Restrictions For Research Use only
Format Liquid
Buffer Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.
General Kamei, Tsutsumi, Taketani et al.: "cDNA cloning and chromosomal localization of neural adhesion molecule NB-3 in human." in: Journal of neuroscience research, Vol. 51, Issue 3, pp. 275-83, 1998 (PubMed).

Lee, Takeda, Kawano et al.: "Expression and regulation of a gene encoding neural recognition molecule NB-3 of the contactin/F3 subgroup in mouse brain." in: Gene, Vol. 245, Issue 2, pp. 253-66, 2000 (PubMed).

Takeda, Akasaka, Lee et al.: "Impaired motor coordination in mice lacking neural recognition molecule NB-3 of the contactin/F3 subgroup." in: Journal of neurobiology, Vol. 56, Issue 3, pp. 252-65, 2003 (PubMed).

Cui, Hu, Tekaya et al.: "NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes." in: The Journal of biological chemistry, Vol. 279, Issue 24, pp. 25858-65, 2004 (PubMed).

Hosts (28), (2), (1)
Reactivities (25), (25), (14), (13), (12), (12)
Applications (17), (12), (10), (6), (4), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (12), (8), (1), (1), (1)
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