GFP antibody

Antigen: GFP

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN271644

Additional Information

Immunogen: Recombinant EGFP.

Cross-Reactivity: Rat (Rattus)

Description: FUNCTION: Energy-transfer acceptor. Its role is to transduce the bluechemiluminescence of the protein aequorin into green fluorescent light by energytransfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activatedphotoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm):488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. SUBUNIT:Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophoreconsisting of modified amino acid residues. The chromophore is formed by autocatalyticbackbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) todidehydrotyrosine. Maturation of the chromophore requires nothing other than molecularoxygen. BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitoustool for making chimeric proteins, where they function as a fluorescent protein tag.Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They havebeen expressed in most known cell types and are used as a noninvasive fluorescentmarker in living cells and organisms. They enable a wide range of applications wherethey have functioned as a cell lineage tracer, reporter of gene expression, or as ameasure of protein-protein interactions. SIMILARITY: Belongs to the GFP family. Alternate Names: anti-Enhanced Green Fluorescent Protein antibody. Related Diseases: Reporter Genes

Specificity: This antibody is known to react with EGFP confirmed by IHC and WB. Species Reactivity: Rat. Localization: Accession Number: EGFP protein , GFP_AEQVI.

Application Details

Application Notes: IHC, Western blot. Recommended to be used at a dilution of 1:1000-1: 5000 for bothapplications. The optimal working dilution should be determined by the end user.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Storage: Store at -20 C. Avoid repetitive freeze/thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Publications

Chalfie, Tu, Euskirchen et al.: "Green fluorescent protein as a marker for gene expression." in: Science (New York, N.Y.), Vol. 263, Issue 5148, pp. 802-5, 1994 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Dardalhon, Noraz, Pollok et al.: "Green fluorescent protein as a selectable marker of fibronectin-facilitated retroviral gene transfer in primary human T lymphocytes." in: Human gene therapy, Vol. 10, Issue 1, pp. 5-14, 1999 (PubMed).