Green Fluorescent Protein (GFP) antibody

Details for Product No. ABIN271644
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Antigen
Reactivity
Aequorea victoria
(338), (31), (11), (8), (4), (2), (2), (1), (1), (1)
Host
Rabbit
(152), (149), (54), (26), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(25), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(301), (156), (105), (67), (66), (38), (37), (31), (30), (29), (13), (11), (10), (10), (8), (4), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN271644
551.76 $
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Immunogen Recombinant EGFP
Specificity This is known to react with EGFP confirmed by IHC and WB.
Purification Whole antisera
Alternative Name GFP
Background FUNCTION: Energy-transfer acceptor. Its role is to transduce the bluechemiluminescence of the protein aequorin into green fluorescent light by energytransfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activatedphotoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm):488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. SUBUNIT:Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophoreconsisting of modified amino acid residues. The chromophore is formed by autocatalyticbackbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) todidehydrotyrosine. Maturation of the chromophore requires nothing other than molecularoxygen. BIOTECHNOLOGY: Fluorescent proteins have become a useful and ubiquitoustool for making chimeric proteins, where they function as a fluorescent protein tag.Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They havebeen expressed in most known cell types and are used as a noninvasive fluorescentmarker in living cells and organisms. They enable a wide range of applications wherethey have functioned as a cell lineage tracer, reporter of gene expression, or as ameasure of protein-protein interactions. SIMILARITY: Belongs to the GFP family. Alternate Names: anti-Enhanced Green Fluorescent Protein antibody. Related Diseases: Reporter Genes
Research Area Tags/Labels
Application Notes IHC-P, Western blot. Recommended to be used at a dilution of 1:1000-1: 5000 for both applications. The optimal working dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Chalfie, Tu, Euskirchen et al.: "Green fluorescent protein as a marker for gene expression." in: Science (New York, N.Y.), Vol. 263, Issue 5148, pp. 802-5, 1994 (PubMed).

Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Dardalhon, Noraz, Pollok et al.: "Green fluorescent protein as a selectable marker of fibronectin-facilitated retroviral gene transfer in primary human T lymphocytes." in: Human gene therapy, Vol. 10, Issue 1, pp. 5-14, 1999 (PubMed).

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