ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) antibody

Antigen: ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5)

Synonyms: ASP, APG5, APG5L, hAPG5, APG5-LIKE, apg5l

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN271645

Additional Information

Alternative name: APG5L / ATG5

Gene ID: 9474

Immunogen: A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogeniccarrier protein was conjugated to the immunogen. Instead, Adjukine B (see theAdjuvants in biosensis' product list) has been used to orchestrate/boost the immuneresponse.

Cross-Reactivity: Human

Description: Autophagy is an important mechanism for nonselective intracellular breakdown wherebycytosol and organelles are encapsulated in vesicles, which are then engulfed anddigested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates toATG12 and associates with isolation membrane to form autophagosomes. Theconjugate detaches from the membrane immediately before or after autophagosomeformation is completed. Conjugation to ATG12 is essential for autophagy, but is notrequired for association with isolation membrane. ATG5 also plays an important role inthe apoptotic process. Its expression is a relatively late event in the apoptotic process,occurring downstream of caspase activity. Alternate Names: anti-Autophagy protein 5 antibody, anti-APG5-like antibody, anti-APG 5 antibody,anti-Apoptosis-specific protein antibody, anti-APG5, biosensis. GENE NAME: ATG5antibody, anti-APG5L antibody, anti-ASP antibody. Related Diseases: Autophagy

Specificity: IHC and WB confirmed the specificity for APG5. Species Reactivity: Human, not yet tested in other species. Localization: Accession Number: ATG5_HUMAN.

Application Details

Application Notes: IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommendedfor these applications. The optimal working dilution should be determined by the enduser.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Restrictions: For Research Use only

Publications

Mizushima, Yoshimori, Ohsumi: "Role of the Apg12 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 35, Issue 5, pp. 553-61, 2003 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).