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Autophagy Related 12 (ATG12) (N-Term) antibody

Details for Product No. ABIN271646
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(96), (42), (40), (12), (12), (12), (4), (1), (1)
(91), (14), (4), (1)
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(87), (62), (54), (13), (13), (12), (10), (5), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN271646
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Immunogen A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Purification IgG purified
Alternative Name APG12 / ATG12
Background FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULARLOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced byalternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to theATG12 family. Alternate Names: anti-Autophagy-related protein 12 antibody, anti-APG12-like antibody, anti-APG 12,biosensis. GENE NAME: ATG12 antibody, anti-APG12 antibody, anti-APG12L antibody. Related Diseases: Autophagy
Gene Symbol: ATG12
Gene ID 9140, 361321
Application Notes Recommended dilutions: Western Blot 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:200-1:1000, Immunohistochemistry 1:200-1:1000, Immunohistochemistry-Paraffin
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water.
Buffer Lyophilized whole serum.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Nishitani, Nemoto et al.: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002 (PubMed).

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