Parkinson Protein 2, E3 Ubiquitin Protein Ligase (PARK2) antibody

Antigen: Parkinson Protein 2, E3 Ubiquitin Protein Ligase (PARK2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN271648

Additional Information

Alternative name: PARK2 / Parkin

Gene ID: 5071, 56816

Immunogen: A synthetic peptide (NSLIKELHHFRILGEE Q) as part of human Parkin conjugated toKLH.

Cross-Reactivity: Human, Rat (Rattus)

Description: Parkinson',s disease is a common neurodegenerative disease with complexclinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible forthe pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role inthe ubiquitin-mediated proteolytic pathway by removal and/or detoxification ofabnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to bethe mechanism underlying pathogenesis of Parkin. Parkin may protect neurons againstalpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, andkainate-induced excitotoxicity. It may play a role in controlling neurotransmittertrafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin alsoregulates cyclin e during neuronal apoptosis and may represent a tumor suppressorgene. Alternate Names: anti-Ubiquitin E3 ligase PRKN antibody, anti-Parkinson juvenile disease protein 2antibody, anti-Parkinson disease protein 2. GENE NAME: PARK2 antibody, anti-PRKNantibody, biosensis. Related Diseases: Alzheimer's/ Parkinson's

Specificity: This antiserum is known to be highly specific for Parkin shown by IHC and WB. Species Reactivity: Reacts with rat and human. Localization: Accession Number: PRKN2_HUMAN.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1:1000,Western Blot 1:2000

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Publications

Kitada, Asakawa, Hattori et al.: "Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism." in: Nature, Vol. 392, Issue 6676, pp. 605-8, 1998 (PubMed).

West, Kapatos, OFarrell et al.: "N-myc regulates parkin expression." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28896-902, 2004 (PubMed).

Wang, Denison, Lai et al.: "Parkin gene alterations in hepatocellular carcinoma." in: Genes, chromosomes & cancer, Vol. 40, Issue 2, pp. 85-96, 2004 (PubMed).

Yao, Gu, Nakamura et al.: "Nitrosative stress linked to sporadic Parkinson's disease: S-nitrosylation of parkin regulates its E3 ubiquitin ligase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10810-4, 2004 (PubMed).

Pigullo, De Luca, Barone et al.: "Mutational analysis of parkin gene by denaturing high-performance liquid chromatography (DHPLC) in essential tremor." in: Parkinsonism & related disorders, Vol. 10, Issue 6, pp. 357-62, 2004 (PubMed).

Kumru, Santamaria, Tolosa et al.: "Rapid eye movement sleep behavior disorder in parkinsonism with parkin mutations." in: Annals of neurology, Vol. 56, Issue 4, pp. 599-603, 2004 (PubMed).