Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) antibody

Antigen: Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2)

Synonyms: TRKB, GP145-TrkB, Tkrb, trkB, AI848316, C030027L06Rik, TRKB1, RATTRKB1, trkb, xtrkb, trkb-b, MGC52547, NTRK2, Trk-B, MGC140769, ntrk2b, ntrk2, MGC99292, MGC145900, gp145-trkb

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN271654

Additional Information

Alternative name: TrkB

Gene ID: 4915, 25054

Immunogen: A synthetic peptide (AFPRLEPNSIDPENC) as part of rat TrkB (aa: 23-36) conjugated toKLH.

Description: FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 andneurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/ormaintenance of the nervous system. This is a tyrosine-protein kinase receptor. Knownsubstrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosinephosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity)and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1.SUBCELLULAR LOCATION: Membrane, single-pass type I membrane protein.ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing.Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms aredifferentially expressed in various cell types. Isoform T2 is primarily expressed inneurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyrprotein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-likeC2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich)repeats. SIMILARITY: Contains 1 protein kinase domain. Alternate Names: anti-BDNF/NT-3 growth factors receptor antibody, anti-Neurotrophic tyrosine kinasereceptor type 2 antibody, anti-TrkB tyrosine kinase antibody,anti-GP145-TrkB/GP95-TrkB antibody, anti-Trk-B. GENE NAME: Ntrk2 antibody,anti-Trkb antibody. Related Diseases: Neurotrophic Receptors

Specificity: Sequence was chosen to avoid cross reactivity with trkA and trkC. Species Reactivity: This antiserum recognises human and rat TrkB. Cross reactivity with other species hasnot yet been tested. Localization: Accession Number: NTRK2_RAT.

Application Details

Application Notes: IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. The optimalworking dilution should be determined by the end user. The sequence of theimmunogen and the conjugate are identical to the one used by Yan et al (see the Refs)and the antiserum appears to have similar characteristics.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Haniu, Talvenheimo, Le et al.: "Extracellular domain of neurotrophin receptor trkB: disulfide structure, N-glycosylation sites, and ligand binding." in: Archives of biochemistry and biophysics, Vol. 322, Issue 1, pp. 256-64, 1995 (PubMed).

Nakagawara, Liu, Ikegaki et al.: "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)." in: Genomics, Vol. 25, Issue 2, pp. 538-46, 1995 (PubMed).

Allen, Dawbarn, Eckford et al.: "Cloning of a non-catalytic form of human trkB and distribution of messenger RNA for trkB in human brain." in: Neuroscience, Vol. 60, Issue 3, pp. 825-34, 1994 (PubMed).

Yan, Radeke, Matheson et al.: "Immunocytochemical localization of TrkB in the central nervous system of the adult rat." in: The Journal of comparative neurology, Vol. 378, Issue 1, pp. 135-57, 1997 (PubMed).

Stoilov, Castren, Stamm: "Analysis of the human TrkB gene genomic organization reveals novel TrkB isoforms, unusual gene length, and splicing mechanism." in: Biochemical and biophysical research communications, Vol. 290, Issue 3, pp. 1054-65, 2002 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).