Myeloperoxidase (MPO) antibody

Antigen: Myeloperoxidase (MPO)

Synonyms: KIAA4033, mKIAA4033, mpo, fj80f04, wu:fj80f04, MPO, LOC100335032

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN271660

Additional Information

Alternative name: Myeloperoxidase

Immunogen: Myeloperoxidase isolated from human polymorphonuclear leucocytes.

Cross-Reactivity: Human

Isotype: IgG

Description: FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It isresponsible for microbicidal activity against a wide range of organisms. In the stimulatedPMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid inphysiologic situations, and other toxic intermediates that greatly enhance PMNmicrobicidal activity. MPO is an important marker for myeloid cells, from thepromyelocyte stage and to the mature forms.CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O.CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O.COFACTOR: Binds 1 calcium ion per heterodimer.COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer.SUBUNIT: Tetramer of two light chains and two heavy chains.SUBCELLULAR LOCATION: Lysosome.ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing.DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD isan autosomal recessive defect that results in disseminated candidiasis.SIMILARITY: Belongs to the peroxidase family. XPO subfamily. Related Diseases: MPO

Specificity: This antiserum reacts with human myeloperoxidase. Species Reactivity: Human.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1:500-1000,Western Blot 1:500-1000

Buffer: Reconstitute in 250 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Research Area: Cancer, Innate Immunity, Enzymes, Metabolism, Immunology

Restrictions: For Research Use only

Publications

Pinkus, Pinkus: "Myeloperoxidase: a specific marker for myeloid cells in paraffin sections." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 4, Issue 6, pp. 733-41, 1992 (PubMed).

Kimura, Ikeda-Saito: "Human myeloperoxidase and thyroid peroxidase, two enzymes with separate and distinct physiological functions, are evolutionarily related members of the same gene family." in: Proteins, Vol. 3, Issue 2, pp. 113-20, 1988 (PubMed).

Weil, Rosner, Reid et al.: "Translocation and rearrangement of myeloperoxidase gene in acute promyelocytic leukemia." in: Science (New York, N.Y.), Vol. 240, Issue 4853, pp. 790-2, 1988 (PubMed).

Lanza: "Clinical manifestation of myeloperoxidase deficiency." in: Journal of molecular medicine (Berlin, Germany), Vol. 76, Issue 10, pp. 676-81, 1998 (PubMed).

Arber, Snyder, Fine et al.: "Myeloperoxidase immunoreactivity in adult acute lymphoblastic leukemia." in: American journal of clinical pathology, Vol. 116, Issue 1, pp. 25-33, 2001 (PubMed).

Kim, Kimura, Ochiai et al.: "Sustained hypomyelination and high serum thyroid hormone in aged black tremor hamster." in: The Japanese journal of veterinary research, Vol. 51, Issue 2, pp. 113-20, 2003 (PubMed).