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Autophagy Related 12 (ATG12) (N-Term) antibody

Details for Product No. ABIN271662
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Antigen
Epitope
N-Term
(25), (6), (6), (6), (6), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(96), (42), (40), (12), (12), (12), (4), (1), (1)
Host
Rabbit
(91), (14), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(87), (62), (54), (13), (13), (12), (10), (5), (1), (1)
Pubmed 3 references available
Quantity 0.5 mg
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN271662
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Immunogen A synthetic peptide corresponding to the N-terminal of human APG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Isotype IgG
Specificity IHC and WB confirmed the specificity for APG12.
Purification Whole antisera
Alternative Name APG12 / ATG12
Background FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULARLOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced byalternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to theATG12 family. Alternate Names: anti-Autophagy-related protein 12 antibody, anti-APG12-like antibody, anti-APG 12,biosensis. GENE NAME: ATG12 antibody, anti-APG12 antibody, anti-APG12L antibody. Related Diseases: Autophagy
Gene Symbol: ATG12
Gene ID 9140, 361321
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Nishitani, Nemoto et al.: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002 (PubMed).

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