anti-ATG5 antibody (ATG5 Autophagy Related 5 Homolog (S. Cerevisiae)) (C-Term)

Details for Product anti-ATG5 Antibody No. ABIN271663
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Antigen
Epitope
C-Term
(46), (24), (12), (12), (11), (9), (8), (6), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(221), (77), (62), (14), (12), (7), (7), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(156), (44), (15), (14), (2)
Clonality
Polyclonal
Conjugate
This ATG5 antibody is un-conjugated
(11), (11), (10), (9), (9), (9), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(178), (113), (110), (26), (22), (12), (12), (10), (6), (6), (5), (2), (1), (1)
Pubmed 2 references available
Quantity 0.5 mg
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Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Isotype IgG
Specificity IHC and wb confirmed the specificity for APG5.
Cross-Reactivity (Details) not yet tested in other species.
Purification affinity purified
Alternative Name APG5L / ATG5 (ATG5 Antibody Abstract)
Background Autophagy is an important mechanism for nonselective intracellular breakdown wherebycytosol and organelles are encapsulated in vesicles, which are then engulfed anddigested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates toATG12 and associates with isolation membrane to form autophagosomes. Theconjugate detaches from the membrane immediately before or after autophagosomeformation is completed. Conjugation to ATG12 is essential for autophagy, but is notrequired for association with isolation membrane. ATG5 also plays an important role inthe apoptotic process. Its expression is a relatively late event in the apoptotic process,occurring downstream of caspase activity. Alternate Names: anti-Autophagy protein 5 antibody, anti-APG5-like antibody, anti-APG 5 antibody,anti-APG5 antibody, anti-Apoptosis-specific protein, biosensis. GENE NAME: ATG5antibody, anti-APG5L antibody, anti-ASP antibody. Related Diseases: Autophagy
Gene Symbol: ATG5
Gene ID 9474
Application Notes IHC-P, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. The optimal working dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Mizushima, Yoshimori, Ohsumi: "Role of the Apg12 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 35, Issue 5, pp. 553-61, 2003 (PubMed).

Validation Images
Catalog No. ABIN271663
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