Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) (N-Term) antibody

Details for Product No. ABIN271665
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Antigen
Epitope
N-Term
(14), (13), (10), (8), (4), (4), (1), (1)
Reactivity
Human
(42), (3), (3), (1)
Host
Rabbit
(34), (4), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(36), (19), (9), (9), (7), (6), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
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Immunogen A synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C (MAP1LC3C, APG8c) conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C (MAP1LC3C, APG8c) protein in Macaca mulatta (monkey) and Canis familiaris (dog).
Specificity IHC and WB confirmed the specificity for MAP1LC3 C (MAP1LC3C, APG8c). This should recognise MAP1LC3 C only and not the other forms MAP1LC3A and MAP1LC3B.
Cross-Reactivity (Details) other species have not yet been tested.
Purification Whole antisera
Alternative Name MAP1LC3C (MAP1LC3C Antibody Abstract)
Background FUNCTION: Probably involved in formation of autophagosomal vacuoles(autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associatewith MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm.LC3-II: Intracytoplasmic membrane, lipid-anchor. LC3-II binds to the autophagicmembranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I.This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipidto form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3family. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3C antibody,anti-Microtubule-associated protein 1 light chain 3 gamma antibody,anti-MAP1A/MAP1B LC3 C antibody, anti-MAP1A/1B light chain 3 C antibody,anti-MAP1 light chain 3-like protein 3 antibody, anti-Autophagy-related protein LC3 Cantibody, anti-Autophagy-related ubiquitin-like modifier LC3 C antibody, anti-APG8c,biosensis. GENE NAME: MAP1LC3C antibody. Related Diseases: Autophagy
Gene Symbol: MAP1LC3C
Gene ID 440738
Application Notes IHC-P, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal working dilution should be determined by the end user.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry 1:100-1:1000, Western Blot 1:100-1:1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

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Catalog No. ABIN271665
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