Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) antibody

Antigen: Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C)

Synonyms: LC3C, MAP1LC3C, MGC154878

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN271665

Additional Information

Alternative name: MAP1LC3C

Gene ID: 440738

Immunogen: A synthetic peptide corresponding to the N-terminal of human MAP1LC3 C(MAP1LC3C, APG8c) conjugated to Blue Carrier Protein has been used as theimmunogen. The peptide is homologous with the corresponding sequence deriven fromMAP1LC3 C (MAP1LC3C, APG8c) protein in Macaca mulatta (monkey) and Canisfamiliaris (dog).

Cross-Reactivity: Human

Description: FUNCTION: Probably involved in formation of autophagosomal vacuoles(autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associatewith MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm.LC3-II: Intracytoplasmic membrane, lipid-anchor. LC3-II binds to the autophagicmembranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I.This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipidto form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3family. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3C antibody,anti-Microtubule-associated protein 1 light chain 3 gamma antibody,anti-MAP1A/MAP1B LC3 C antibody, anti-MAP1A/1B light chain 3 C antibody,anti-MAP1 light chain 3-like protein 3 antibody, anti-Autophagy-related protein LC3 Cantibody, anti-Autophagy-related ubiquitin-like modifier LC3 C antibody, anti-APG8c,biosensis. GENE NAME: MAP1LC3C antibody. Related Diseases: Autophagy

Specificity: IHC and WB confirmed the specificity for MAP1LC3 C (MAP1LC3C, APG8c). Thisantibody should recognise MAP1LC3 C only and not the other forms MAP1LC3A andMAP1LC3B. Species Reactivity: Human, other species have not yet been tested. Localization: Accession Number: MLP3C_HUMAN.

Application Details

Application Notes: IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommendedfor these applications. The optimal working dilution should be determined by the enduser.

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Restrictions: For Research Use only

Publications

Tanida, Ueno, Kominami: "LC3 conjugation system in mammalian autophagy." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 12, pp. 2503-18, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).