Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody

Details for Product No. ABIN271671
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Antigen
Epitope
N-Term
(25), (22), (13), (12), (11), (10), (10), (7), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(178), (85), (74), (42), (25), (25), (15), (12), (3), (3), (1), (1), (1)
Host
Rabbit
(156), (18), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (10), (10), (10), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(141), (64), (59), (39), (31), (30), (16), (15), (14), (10), (6), (5), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN271671
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Immunogen A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen.
Specificity IHC, WB and ELISA confirmed the specificity for MAP1LC3 A (MAP1LC3A, APG8a).
Cross-Reactivity (Details) Predicted cross-reactivity due to antigen sequence identity: Mouse. Other species not yet tested.
Purification Whole antisera
Alternative Name LC3A
Background FUNCTION: Probably involved in formation of autophagosomal vacuoles(autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associatewith MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm.LC3-II: Intracytoplasmic membrane, lipid-anchor. LC3-II binds to the autophagicmembranes. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form thecytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 andconjugated to phospholipid to form the membrane-bound form, LC3-II. SIMILARITY:Belongs to the MAP1 LC3 family. Alternate Names: anti-Microtubule-associated proteins 1A/1B light chain 3A antibody, anti-MAP1A/MAP1BLC3 A antibody, anti-MAP1A/1B light chain 3 A antibody, anti-MAP1 light chain 3-likeprotein 1 antibody, anti-Microtubule-associated protein 1 light chain 3 alpha antibody,anti-Autophagy-related protein LC3 A antibody, anti-Autophagy-related ubiquitin-likemodifier LC3 A antibody, anti-APG8a, biosensis. GENE NAME: MAP1LC3A antibody. Related Diseases: Autophagy
Gene Symbol: MAP1LC3A
Gene ID 84557, 362245
Application Notes Immunofluorescence and WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in IHC-P.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry 1:100-1:1000, Western Blot 1:100-1:1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration. Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

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