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ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) antibody

Details for Product No. ABIN271683
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Antigen
Reactivity
Human
(98), (42), (40), (25), (25), (24), (24), (1), (1), (1)
Host
Rabbit
(78), (17), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(62), (41), (24), (20), (11), (10), (10), (7), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN271683
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Immunogen A synthetic peptide corresponding to a central region of the human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Cross-Reactivity (Details) The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey),Canis familiaris (dog).
Purification Whole antisera
Alternative Name APG3 / ATG3
Background FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier proteinconjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-likeenzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-likeenzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PEconjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. Thecomplex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 toATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 namedisoformsproduced by alternative splicing. TISSUE SPECIFICITY: Widely expressed,with a highest expression in heart, skeletal muscle, kidney, liver and placenta.SIMILARITY: Belongs to the ATG3 family. Alternate Names: biosensis. Related Diseases: Autophagy
Gene Symbol: ATG3
Gene ID 64422, 171415
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:150-1:3000, Immunohistochemistry 1:150-1:3000, Immunohistochemistry-Paraffin 1:150-1:3000, Western Blot 1:150-1:3000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water.
Buffer Lyophilized whole serum.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

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