Glial Fibrillary Acidic Protein (GFAP) antibody

Details for Product No. ABIN271686
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Antigen
Reactivity
Human
(435), (149), (146), (82), (63), (17), (15), (12), (11), (9), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(274), (237), (24), (10), (3), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(21), (16), (15), (8), (8), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(389), (213), (178), (174), (117), (109), (73), (41), (30), (10), (5), (5), (5), (4), (3), (2), (1), (1), (1)
Pubmed 10 references available
Quantity 0.25 mg
Shipping to United States (Change)
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Catalog No. ABIN271686
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Immunogen Native GFAP extracted from rat spinal cord .
Isotype IgG
Specificity GFAP
Cross-Reactivity (Details) Expected to react with cat, dog,sheep
Purification Protein G purified
Alternative Name GFAP
Background Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filamentprotein family. It is heavily, and specifically, expressed in astrocytes and certain otherastroglia in the central nervous system, in satellite cells in peripheral ganglia, and in nonmyelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequentlystrongly express GFAP. Antibodies to GFAP are therefore very useful as markers ofastrocytic cells. In addition many types of brain tumor, presumably derived fromastrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium,Kupffer cells of the liver, in some cells in salivary tumors and has been reported inerythrocytes. Alternate Names: anti-Astrocyte antibody, anti-Glial Fibrillary Acidic Protein antibody, anti-FLJ45472antibody. Related Diseases: Developmental CNS
Gene Symbol: GFAP
Gene ID 2670, 14580, 24387
Research Area Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
Application Notes Immunohistochemistry-paraffin and ELISA. A dilution of 1:500-1:1000 is recommended. This antiserum works superbly for staining of paraffin-embedded tissue sections fixed in formalin, frozen sections and cell cytospins. Proteinase-K pre-treatment of tissues has been shown to produce most specific pattern of staining with negligible background. Occasionally, antigen retrieval by microwave (heat retrieval of the epitope) can produce some non-specific staining. The optimal dilution should be determined by the end user.
Recommended dilutions: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin 1:500-1:1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 250 µL of sterile water.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Brenner, Lampel, Nakatani et al.: "Characterization of human cDNA and genomic clones for glial fibrillary acidic protein." in: Brain research. Molecular brain research, Vol. 7, Issue 4, pp. 277-86, 1990 (PubMed).

Reeves, Helman, Allison et al.: "Molecular cloning and primary structure of human glial fibrillary acidic protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 13, pp. 5178-82, 1989 (PubMed).

Brenner, Johnson, Boespflug-Tanguy et al.: "Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease." in: Nature genetics, Vol. 27, Issue 1, pp. 117-20, 2001 (PubMed).

Aoki, Haginoya, Munakata et al.: "A novel mutation in glial fibrillary acidic protein gene in a patient with Alexander disease." in: Neuroscience letters, Vol. 312, Issue 2, pp. 71-4, 2001 (PubMed).

Sawaishi, Yano, Takaku et al.: "Juvenile Alexander disease with a novel mutation in glial fibrillary acidic protein gene." in: Neurology, Vol. 58, Issue 10, pp. 1541-3, 2002 (PubMed).

Nielsen, Holm, Johansen et al.: "A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29983-91, 2002 (PubMed).

Singh, Nielsen, Johansen et al.: "Genetic polymorphism and sequence evolution of an alternatively spliced exon of the glial fibrillary acidic protein gene, GFAP." in: Genomics, Vol. 82, Issue 2, pp. 185-93, 2003 (PubMed).

Brockmann, Meins, Taubert et al.: "A novel GFAP mutation and disseminated white matter lesions: adult Alexander disease?" in: European neurology, Vol. 50, Issue 2, pp. 100-5, 2003 (PubMed).

Stumpf, Masson, Duquette et al.: "Adult Alexander disease with autosomal dominant transmission: a distinct entity caused by mutation in the glial fibrillary acid protein gene." in: Archives of neurology, Vol. 60, Issue 9, pp. 1307-12, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

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