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clathrin, Heavy Chain (Hc) (CLTC) (AA 619-638) antibody

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AA 619-638
(16), (15), (13), (8), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Rat (Rattus)
(58), (27), (23), (9), (3), (1), (1), (1)
(40), (22), (6), (1)
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(48), (31), (20), (19), (12), (11), (11), (9), (7), (4), (1), (1), (1)
Pubmed 6 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.
Specificity This has been shown to be specific for clathrin.
Purification Whole antisera
Alternative Name Clathrin heavy chain (CLTC Antibody Abstract)
Background Membrane vesicle formation is a process required for the endocytosis and biosynthesisof various secreted and membrane bound proteins. Clathrin is a protein whichassembles into a polyhedral network on the cell membrane as the membraneinvaginates, forming a coated pit which is essential to endocytosis. Clathrin is composedof three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains whichcombine to create a distinct three-legged triskelion. It is this morphology which allowsClathrin to form its unique polyhedral network. Alternate Names: anti-Clathrin heavy chain antibody, anti-Clathrin heavy chain 1 antibody, anti-CLH-17.GENE NAME: CLTC antibody. Related Diseases: Intracellular Trafficking
Gene Symbol: AP1B1
Gene ID 162
Research Area Organelles, Transporters
Application Notes Immunohistochemistry - paraffin or frozen and Western Blot. This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Immunoprecipitation was reported in scientific literature. Antigen retrieval has been used in testing but may not be necessary.
Recommended dilutions: Immunohistochemistry, Immunohistochemistry-Frozen 1: 100 to 1: 4000, Immunohistochemistry-Paraffin 1: 100 to 1: 4000, Immunoprecipitation, Western Blot 1: 1000 to 1: 8000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Chi, Valencia, Hu et al.: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

ter Haar, Musacchio, Harrison et al.: "Atomic structure of clathrin: a beta propeller terminal domain joins an alpha zigzag linker." in: Cell, Vol. 95, Issue 4, pp. 563-73, 1998 (PubMed).

Nomura, Miyajima, Sazuka et al.: "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature ..." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 1, Issue 1, pp. 27-35, 1995 (PubMed).

Dodge, Kovalszky, McBride et al.: "Human clathrin heavy chain (CLTC): partial molecular cloning, expression, and mapping of the gene to human chromosome 17q11-qter." in: Genomics, Vol. 11, Issue 1, pp. 174-8, 1992 (PubMed).

Catalog No. ABIN271687

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