clathrin, Heavy Chain (Hc) (CLTC) antibody

Details for Product No. ABIN271687
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Antigen
Synonyms CHC, CHC17, CLH-17, CLTCL2, Hc, 3110065L21Rik, R74732, CLTC, Chc
Reactivity
Rat (Rattus)
(29), (23), (18), (18), (14), (13), (1), (1), (1), (1)
Host
Rabbit
(18), (16), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(25), (10), (9), (8), (7), (7), (5), (4), (3), (2)
Pubmed 6 references available
Catalog no. ABIN271687
Quantity 0.1 mL
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Immunogen A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen.The peptide is homologous with the corresponding sequence derived from clathrinprotein in mouse, human and bovine.
Specificity This antibody has been shown to be specific for clathrin. Species Reactivity: Reacts with Rat. Localization: Accession Number: CLH_RAT , CLH_MOUSE , CLH_HUMAN , CLH_BOVIN.
Cross-Reactivity Rat (Rattus)
Alternative Name Clathrin heavy chain
Background Membrane vesicle formation is a process required for the endocytosis and biosynthesisof various secreted and membrane bound proteins. Clathrin is a protein whichassembles into a polyhedral network on the cell membrane as the membraneinvaginates, forming a coated pit which is essential to endocytosis. Clathrin is composedof three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains whichcombine to create a distinct three-legged triskelion. It is this morphology which allowsClathrin to form its unique polyhedral network. Alternate Names: anti-Clathrin heavy chain antibody, anti-Clathrin heavy chain 1 antibody, anti-CLH-17.GENE NAME: CLTC antibody. Related Diseases: Intracellular Trafficking
Research Area Organelles, Transporters
Application Notes Suggested working dilutions: immunohistochemistry 1: 100 to 1: 4000,Western Blot 1: 1000 to 1: 8000,Immunohistochemistry-Paraffin 1: 100 to 1: 4000,Immunohistochemistry-Frozen 1: 100 to 1: 4000
Restrictions For Research Use only
Format Liquid
Buffer Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.
Storage 4 °C
General Dodge, Kovalszky, McBride et al.: "Human clathrin heavy chain (CLTC): partial molecular cloning, expression, and mapping of the gene to human chromosome 17q11-qter." in: Genomics, Vol. 11, Issue 1, pp. 174-8, 1992 (PubMed).

Nomura, Miyajima, Sazuka et al.: "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature..." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 1, Issue 1, pp. 27-35, 1995 (PubMed).

ter Haar, Musacchio, Harrison et al.: "Atomic structure of clathrin: a beta propeller terminal domain joins an alpha zigzag linker." in: Cell, Vol. 95, Issue 4, pp. 563-73, 1998 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Chi, Valencia, Hu et al.: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).

Hosts (18), (16), (3)
Reactivities (29), (23), (18), (18), (14), (13), (1), (1), (1), (1)
Applications (25), (10), (9), (8), (7), (7), (5), (4), (3), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (2), (1), (1), (1), (1), (1), (1), (1)
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