clathrin, Heavy Chain (Hc) (CLTC) antibody

Antigen: clathrin, Heavy Chain (Hc) (CLTC)

Synonyms: Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN271687

Additional Information

Alternative name: Clathrin heavy chain

Immunogen: A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen.The peptide is homologous with the corresponding sequence derived from clathrinprotein in mouse, human and bovine.

Cross-Reactivity: Rat (Rattus)

Description: Membrane vesicle formation is a process required for the endocytosis and biosynthesisof various secreted and membrane bound proteins. Clathrin is a protein whichassembles into a polyhedral network on the cell membrane as the membraneinvaginates, forming a coated pit which is essential to endocytosis. Clathrin is composedof three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains whichcombine to create a distinct three-legged triskelion. It is this morphology which allowsClathrin to form its unique polyhedral network. Alternate Names: anti-Clathrin heavy chain antibody, anti-Clathrin heavy chain 1 antibody, anti-CLH-17.GENE NAME: CLTC antibody. Related Diseases: Intracellular Trafficking

Specificity: This antibody has been shown to be specific for clathrin. Species Reactivity: Reacts with Rat. Localization: Accession Number: CLH_RAT , CLH_MOUSE , CLH_HUMAN , CLH_BOVIN.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 1: 100 to 1: 4000,Western Blot 1: 1000 to 1: 8000,Immunohistochemistry-Paraffin 1: 100 to 1: 4000,Immunohistochemistry-Frozen 1: 100 to 1: 4000

Buffer: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Research Area: Organelles, Transporters

Restrictions: For Research Use only

Publications

Dodge, Kovalszky, McBride et al.: "Human clathrin heavy chain (CLTC): partial molecular cloning, expression, and mapping of the gene to human chromosome 17q11-qter." in: Genomics, Vol. 11, Issue 1, pp. 174-8, 1992 (PubMed).

Nomura, Miyajima, Sazuka et al.: "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 1, Issue 1, pp. 27-35, 1995 (PubMed).

ter Haar, Musacchio, Harrison et al.: "Atomic structure of clathrin: a beta propeller terminal domain joins an alpha zigzag linker." in: Cell, Vol. 95, Issue 4, pp. 563-73, 1998 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Chi, Valencia, Hu et al.: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).