anti-gamma 1 Adaptin antibody (Adaptor-Related Protein Complex 1, gamma 1 Subunit) (AA 5-24)

Details for Product anti-AP1G1 Antibody No. ABIN271688
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Antigen
Epitope
AA 5-24
(12), (7), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(60), (40), (40), (7), (3), (3), (1), (1), (1)
Host
Rabbit
(46), (14), (11)
Clonality
Polyclonal
Conjugate
This gamma 1 Adaptin antibody is un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(45), (20), (20), (13), (11), (10), (7), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
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Immunogen A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Specificity This has been shown to be specific for AP-1 complex subunit gamma-1.
Purification Whole antisera
Alternative Name AP1 complex subunit gamma-1 (AP1G1 Antibody Abstract)
Background Adaptins are important components of clathrin-coated vesicles transportingligand-receptor complexes from the plasma membrane or from the trans-Golgi networkto lysosomes. The adaptin family of proteins is composed of four classes of moleculesnamed alpha, beta, beta prime and gamma adaptins. Adaptins, together with mediumand small subunits, form a heterotetrameric complex called an adaptor, whose role is topromote the formation of clathrin-coated pits and vesicles. The protein encoded by thisgene is a gamma-adaptin protein and it belongs to the adaptor complexes largesubunits family. Alternate Names: anti-Adapter-related protein complex 1 gamma-1 subunit Gamma-adaptin antibody,anti-Adaptor protein complex AP-1 gamma-1 subunit antibody, anti-Golgi adaptorHA1/AP1 adaptin subunit gamma-1 antibody, anti-Clathrin assembly protein complex 1gamma-1 large chain antibody, anti-GENE NAME: AP1G1 antibody, anti-ADTGantibody, anti-CLAPG1 antibody. Related Diseases: Intracellular Trafficking
Gene Symbol: AP1G1
Gene ID 164, 171494
Application Notes Immunohistochemistry - paraffin or frozen and Western Blot. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions are 1: 200 to 1: 2000 depending on tissue and detection method.
Recommended dilutions: Western Blot 1: 1000 to 1: 4000, Immunohistochemistry-Paraffin 1: 200 to 1: 2000, Immunohistochemistry
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add diH20 to desired concentration.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Deneka, Neeft, Popa et al.: "Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes." in: The EMBO journal, Vol. 22, Issue 11, pp. 2645-57, 2003 (PubMed).

Nogi, Shiba, Kawasaki et al.: "Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain." in: Nature structural biology, Vol. 9, Issue 7, pp. 527-31, 2002 (PubMed).

Takatsu, Sakurai, Shin et al.: "Identification and characterization of novel clathrin adaptor-related proteins." in: The Journal of biological chemistry, Vol. 273, Issue 38, pp. 24693-700, 1998 (PubMed).

Peyrard, Parveneh, Lagercrantz et al.: "Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG)." in: Genomics, Vol. 50, Issue 2, pp. 275-80, 1998 (PubMed).

Robinson: "Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2319-26, 1991 (PubMed).

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Catalog No. ABIN271688
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