Ubiquitin (AA 48-60) antibody

Details for Product No. ABIN271710
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Antigen
Epitope
AA 48-60
(13), (8), (7), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Sheep (Ovine)
(151), (109), (57), (57), (15), (15), (9), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host
Sheep
(138), (116), (3), (2), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(184), (128), (82), (66), (37), (32), (24), (23), (20), (10), (2), (1)
Pubmed 5 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN271710
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Immunogen A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ubiquitin conjugated to Blue carrier protein has been used as the immunogen.
Specificity This antiserum is highly specific for ubiquitin and ubiquitin conjugates.
Purification Whole antisera
Background Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has anATP dependent role in the targeting of proteins for proteolytic degradation. To performthis function, the protein to be degraded is first covalently attached to the C terminus ofubiquitin, and the ubiquitinated complex is then recognized by a complex of degradativeenzymes. Interestingly, ubiquitin also becomes covalently bonded to many types ofpathological inclusions, which appear to be resistant to normal degradation. Therefore,ubiquitin antibodies are very useful for studies of these inclusions. For example, theneurofibrillary tangles and paired helical filaments diagnostic of Alzheimer',sdisease, Lewy bodies seen in Parkinson',s disease, and Pick bodies found inPick',s disease are all heavily ubiquitinated and can be readily visualized withubiquitin antibodies. Alternate Names: anti-60S ribosomal protein L40 antibody, anti-HUBCEP52 antibody, anti-RPL40antibody, anti-Ubiquitin 52 amino acid fusion protein antibody, anti-Ubiquitin A52 residueribosomal protein fusion product 1 antibody, anti-Ubiquitin carboxyl extension protein 52antibody, anti-Ubiquitin CEP52 antibody. Related Diseases: Ubiquitin & Related
Gene Symbol: AUP1
Gene ID 550, 50522
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids
Application Notes Recommended dilutions: Immunohistochemistry 1:500-1:6000, Immunohistochemistry-Frozen 1:500-1:6000, Immunohistochemistry-Paraffin 1:500-1:6000, Western Blot 1:500-1:6000
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Buffer Lyophilized whole serum.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Wilkinson, Audhya: "Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly." in: The Journal of biological chemistry, Vol. 256, Issue 17, pp. 9235-41, 1981 (PubMed).

Coux, Tanaka, Goldberg: "Structure and functions of the 20S and 26S proteasomes." in: Annual review of biochemistry, Vol. 65, pp. 801-47, 1996 (PubMed).

Jang, Weber, Bashir et al.: "Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13." in: Genomics, Vol. 36, Issue 2, pp. 366-8, 1997 (PubMed).

Schwartz, Ciechanover: "The ubiquitin-proteasome pathway and pathogenesis of human diseases." in: Annual review of medicine, Vol. 50, pp. 57-74, 1999 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

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