Glutathione Peroxidase 1 (GPX1) antibody

Details for Product No. ABIN271731
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Antigen
Synonyms Gpx, CGPx, GPx-1, GSHPx-1, AI195024, AL033363, GPXD, GSHPX1, GSHPx, gshpx1, GPX1, gpx1, cb690, zgc:92737, zgc:103683
Reactivity
Human
(77), (34), (27), (23), (14), (12), (12), (2), (1)
Host
Sheep
(46), (23), (17), (12), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(67), (54), (28), (19), (17), (16), (11), (10), (10), (6), (3)
Pubmed 5 references available
Catalog no. ABIN271731
Quantity 0.1 mL
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Immunogen A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin hasbeen used as the immunogen.
Specificity This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1)protein. Species Reactivity: Human and rat, other species have not yet been tested. Localization: Accession Number: GPX1_HUMAN.
Cross-Reactivity Human, Rat (Rattus)
Alternative Name Glutathione peroxidase 1 / GPX1
Background FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown.CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O.SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY:Belongs to the glutathione peroxidase family. Alternate Names: anti-GSHPx-1 antibody, anti-GPx-1 antibody, anti-Cellular glutathione peroxidase.GENE NAME: GPX1 antibody, biosensis. Related Diseases: Antioxidant Enzymes
Gene ID 2876, 24404
Application Notes IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffinembedded tissues. Antigen retrieval has been used in testing but may not be necessary.Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000depending on tissue and detection method. For western blotting a dilution range of 1:10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 isrecommended. The optimal dilution should be determined by the end user. Thisantiserum has extremely high titre, it stains human and rat brain microglia intensely andto some extent also stains neurons. Other tissues have not yet been tested. On Westernblotting under reducing conditions recognises a 22kD protein in human brain tissue andSigma glutathione peroxidase (G-4013) isolated from human erythrocytes.
Restrictions For Research Use only
Format Liquid
Buffer Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.
General Chada, Le Beau, Casey et al.: "Isolation and chromosomal localization of the human glutathione peroxidase gene." in: Genomics, Vol. 6, Issue 2, pp. 268-71, 1990 (PubMed).

Mullenbach, Tabrizi, Irvine et al.: "Sequence of a cDNA coding for human glutathione peroxidase confirms TGA encodes active site selenocysteine." in: Nucleic acids research, Vol. 15, Issue 13, pp. 5484, 1987 (PubMed).

Sukenaga, Ishida, Takeda et al.: "cDNA sequence coding for human glutathione peroxidase." in: Nucleic acids research, Vol. 15, Issue 17, pp. 7178, 1987 (PubMed).

Ishida, Morino, Takagi et al.: "Nucleotide sequence of a human gene for glutathione peroxidase." in: Nucleic acids research, Vol. 15, Issue 23, pp. 10051, 1988 (PubMed).

Forsberg, de Faire, Morgenstern: "Low yield of polymorphisms from EST blast searching: analysis of genes related to oxidative stress and verification of the P197L polymorphism in GPX1." in: Human mutation, Vol. 13, Issue 4, pp. 294-300, 1999 (PubMed).

Hosts (46), (23), (17), (12), (1)
Reactivities (77), (34), (27), (23), (14), (12), (12), (2), (1)
Applications (67), (54), (28), (19), (17), (16), (11), (10), (10), (6), (3)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (9), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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