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Peroxiredoxin 6 (PRDX6) antibody
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
|11 references available|
|Price||Product not available in this region.|
|Alternative name||Peroxiredoxin-6 (PRDX6)|
|Gene ID||9588, 11758, 94167|
|Immunogen||Rat recombinant Peroxiredoxin-6.|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and shortchain organic, fatty acid, and phospholipid hydroperoxides. May play a role in theregulation of phospholipid turnover as well as in protection against oxidative injury.CATALYTIC ACTIVITY: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. SUBUNIT:Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm.Lysosome. Note=Also found in lung secretory organelles. MISCELLANEOUS: Theactive site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly reactwith a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitanthomodimer formation. The enzyme may be subsequently regenerated by reduction ofthe disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated byoveroxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs tothe ahpC/TSA family. Rehydrin subfamily. Alternate Names: anti-Non-selenium glutathione peroxidase antibody, anti-antioxidant protein 2 antibody,anti-1-Cys peroxiredoxin antibody, anti-1-Cys PRX antibody, anti-Acidiccalcium-independent phospholipase A2 antibody, anti-NSGPx antibody,anti-Thiol-specific antioxidant protein, biosensis. GENE NAME: Prdx6 antibody,anti-Aipla2 antibody, anti-Aop2 antibody, anti-Tsa antibody. Related Diseases: Antioxidant Enzymes|
|Specificity||This antibody has been shown to be specific for Peroxiredoxin-6 protein. Species Reactivity: Reacts with Rat, Human and Mouse. Localization: Accession Number: PRDX6_RAT , PRDX6_HUMAN , PRDX6_MOUSE.|
|Synonyms||PRX, p29, AOP2, 1-Cys, NSGPx, aiPLA2, KIAA0106, MGC46173, GPx, Aop2, CP-3, Ltw4, Ltw-4, ORF06, Prdx5, Brp-12, Lvtw-4, 1-cysPrx, AA690119, Aop2-rs3, Prdx6-rs3, mKIAA0106, 9430088D19Rik, AOPP, PrxV, Pmp20, Prdx6, AOEB166, PRDX6, MGC73360, zgc:73360, aop2, prx6, MGC76137, MGC64582, prdx6, MGC64522|
|Application Notes||Suggested working dilutions: ELISA 1:1000 to 1:2000,immunohistochemistry 1:100 to 1:1000,Western Blot 1:500 to 1:2000,Immunohistochemistry-Paraffin 1:100 to 1:1000,Immunohistochemistry-Frozen 1:100 to 1:1000|
|Buffer||Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Preservative: No Preservative.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
Nagase, Miyajima, Tanaka et al.: "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 2, Issue 1, pp. 37-43, 1995 (PubMed).
Kim, Sundaresh, Feinstein et al.: "Identification of a human cDNA clone for lysosomal type Ca2+-independent phospholipase A2 and properties of the expressed protein." in: The Journal of biological chemistry, Vol. 272, Issue 4, pp. 2542-50, 1997 (PubMed).
Frank, Munz, Werner: "The human homologue of a bovine non-selenium glutathione peroxidase is a novel keratinocyte growth factor-regulated gene." in: Oncogene, Vol. 14, Issue 8, pp. 915-21, 1997 (PubMed).
Kang, Baines, Rhee: "Characterization of a mammalian peroxiredoxin that contains one conserved cysteine." in: The Journal of biological chemistry, Vol. 273, Issue 11, pp. 6303-11, 1998 (PubMed).
Choi, Kang, Yang et al.: "Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution." in: Nature structural biology, Vol. 5, Issue 5, pp. 400-6, 1998 (PubMed).
Power, Nicholas: "Immunohistochemical localization and characterization of a rat Clara cell 26-kDa protein (CC26) with similarities to glutathione peroxidase and phospholipase A2." in: Experimental lung research, Vol. 25, Issue 5, pp. 379-92, 1999 (PubMed).
Chen, Dodia, Feinstein et al.: "1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28421-7, 2000 (PubMed).
Wagner, Luche, Penna et al.: "A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress." in: The Biochemical journal, Vol. 366, Issue Pt 3, pp. 777-85, 2002 (PubMed).
Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).
Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).
Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).
|Hosts||Rabbit (26), Mouse (14), Sheep (1)|
|Reactivities||Human (33), Rat (Rattus) (14), Mouse (Murine) (8), Chicken (2), Cow (Bovine) (2), Pig (Porcine) (2), Xenopus laevis (2), Zebrafish (1)|
|Applications||Western Blotting (WB) (37), ELISA (30), Immunohistochemistry (IHC) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunocytochemistry (ICC) (5), Immunofluorescence (IF) (5), Immunoprecipitation (IP) (5), Flow Cytometry (FACS) (2)|
|Conjugates||Biotin (2), FITC (1)|
|Epitopes||C-Term (3), Center (1), Internal Region (1)|