Dynamitin antibody

Catalog No. ABIN271764

Product Details anti-Dynamitin Antibody

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p50 Dynamitin protein. (region surrounding Leu371)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Alternative Name: Dynactin Subunit 2 (DCTN2 Products)

Synonyms: DCTN2 antibody, dctn2 antibody, dctn50 antibody, dynamitin antibody, rbp50 antibody, CG8269 antibody, DMN antibody, Dmel\\CG8269 antibody, P50 antibody, dDyn antibody, dmn antibody, dyna antibody, l(2)k16109 antibody, l(2)k16218 antibody, p50 antibody, p50/Dmn antibody, p50/dynamitin antibody, 2310042E05Rik antibody, C130077D06Rik antibody, C87049 antibody, DCTN-50 antibody, GMP23-48K antibody, RBP50 antibody, DCTN50 antibody, DYNAMITIN antibody, zgc:63867 antibody, dynactin subunit 2 antibody, dynactin subunit 2 S homeolog antibody, Dynactin 2, p50 subunit antibody, dynactin 2 antibody, dynactin 2 (p50) antibody, dynactin subunit 2 L homeolog antibody, DCTN2 antibody, dctn2.S antibody, DCTN2-p50 antibody, dctn2 antibody, Dctn2 antibody, dctn2.L antibody

Background: Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This , is against the full length p50 dynamitin.Synonyms: 50 kDa dynein-associated polypeptide, DCTN2, DCTN50, Dynactin 2, Dynactin complex 50 kDa subunit, RBP50, p50 dynamitin

Molecular Weight: approx. 44 kDa

Gene ID: 10540

NCBI Accession: NP_006391

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization