WNK1 antibody

Catalog No. ABIN271862

Product Details anti-WNK1 Antibody

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of WNK1 protein. (region surrounding Tyr52)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Alternative Name: WNK1 (WNK1 Products)

Synonyms: HSAN2 antibody, HSN2 antibody, KDP antibody, PRKWNK1 antibody, PSK antibody, p65 antibody, WNK1 antibody, kdp antibody, psk antibody, hsn2 antibody, hsan2 antibody, pha2c antibody, prkwnk1 antibody, 6430573H23Rik antibody, EG406236 antibody, Hsn2 antibody, Prkwnk1 antibody, mKIAA0344 antibody, ATWNK1 antibody, T9J14.14 antibody, T9J14_14 antibody, ZIK4 antibody, with no lysine (K) kinase 1 antibody, WNK lysine deficient protein kinase 1 antibody, WNK lysine deficient protein kinase 1 L homeolog antibody, serine/threonine-protein kinase WNK1 antibody, with no lysine (K) kinase 1 antibody, WNK1 antibody, wnk1.L antibody, wnk1 antibody, LOC100056745 antibody, Wnk1 antibody

Background: The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250 - 300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95 % homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion.Synonyms: Erythrocyte 65 kDa protein, KDP, KIAA0344, Kinase deficient protein, PRKWNK1, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, Serine/threonine-protein kinase WNK1, p65

Molecular Weight: approx. 230 kDa

Gene ID: 65125

NCBI Accession: NP_001171914

UniProt: Q9H4A3