Ephrin B1 antibody
-
- Target See all Ephrin B1 (EFNB1) Antibodies
- Ephrin B1 (EFNB1)
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Ephrin B1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Ephrin-B protein. (region surrounding Gln324)
- Cross-Reactivity (Details)
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: Ephrin-B2 - Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product EFNB1 Primary Antibody
-
-
- Application Notes
-
ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- Ephrin B1 (EFNB1)
- Alternative Name
- Ephrin-B1 (EFNB1 Products)
- Synonyms
- EFNB1 antibody, LERK2 antibody, Cek5-L antibody, EFL-3 antibody, Elk-L antibody, Epl2 antibody, Eplg2 antibody, LERK-2 antibody, Lerk2 antibody, Stra1 antibody, CFND antibody, CFNS antibody, EFL3 antibody, EPLG2 antibody, cfnd antibody, cfns antibody, efl3 antibody, efnb1-A antibody, ephrinB1 antibody, eplg2 antibody, lerk2 antibody, ephrin B1 antibody, ephrin-B1 antibody, ephrin-B1 L homeolog antibody, EFNB1 antibody, Efnb1 antibody, efnb1 antibody, efnb1.L antibody
- Background
- Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2
- Molecular Weight
- approx. 59 kDa
- Gene ID
- 1947
- NCBI Accession
- NP_004420
- UniProt
- P98172
- Pathways
- RTK Signaling
-