SAE1 antibody

Catalog No. ABIN271943

Product Details anti-SAE1 Antibody

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of AOS1 protein. (region surrounding Leu247)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IF: 1: 50-1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1 / AOS1 (SAE1 Products)

Synonyms: AOS1 antibody, HSPC140 antibody, SUA1 antibody, UBLE1A antibody, 2400010M20Rik antibody, 2610044L12Rik antibody, AL033372 antibody, AW743391 antibody, D7Ertd177e antibody, Sua1 antibody, Uble1a antibody, Aos1p antibody, Sua1p antibody, aos antibody, sae2a antibody, uble1a antibody, wu:fa28b04 antibody, zgc:86633 antibody, SUMO1 activating enzyme subunit 1 antibody, SUMO1 activating enzyme subunit 1 L homeolog antibody, SAE1 antibody, Sae1 antibody, sae1.L antibody, sae1 antibody

Background: Proteolytic degradation by the ubiquitin (Ub) system is essential for normal cell cycle progression, cellular differentiation and stress responses. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteosome. Ubiquitin-like 1-activating enzyme E1A, also designated SUMO-1-activating enzyme or AOS-1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. AOS-1, which is a dimeric enzyme, functions as a UBLI E1 ligase, mediating the ATP-dependent activation of UBL1. It can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A

Molecular Weight: approx. 38 kDa

Gene ID: 10055

NCBI Accession: NP_005491

UniProt: Q9UBE0