MYL12B antibody

Catalog No. ABIN272037

Product Details anti-MYL12B Antibody

Target: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL12B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of MRLC2 protein. (region surrounding Pro13)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MYL12B

Target: MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))

Alternative Name: MYL12B (MYL12B Products)

Synonyms: Mrlc2 antibody, Mrlcb antibody, Mylc2b antibody, MLC-B antibody, MRLC2 antibody, 1500001M02Rik antibody, C77744 antibody, RLC-B antibody, MYL12B antibody, mlc-b antibody, mrlc2 antibody, mrlcb antibody, myl12b antibody, myosin light chain 12B antibody, myosin, light chain 12B, regulatory antibody, Myosin regulatory light chain 2 antibody, myosin regulatory light chain 2 antibody, myosin regulatory light chain 12B antibody, myosin light chain 12B L homeolog antibody, Myl12b antibody, MYL12B antibody, mlc-2 antibody, MLC-2 antibody, LOC102175348 antibody, LOC101105123 antibody, LOC100062440 antibody, myl12b.L antibody

Background: Myosin is a 480 kD protein that interacts with actin in muscle and non muscle cells (8). Conventional myosin contains two identical heavy chains (MHC-200kD each) and four light chains (MLC-15-26 kD). Conventional myosin molecules consist of two major regions: tail (rod) and globular heads, they aggregate into filaments through the tail region and interact with actin and with adenosine triphosphate (ATP) through the head region. Multiple forms of myosin heavy chains exist in skeletal, smooth and cardiac muscle and in non-muscle tissues. A spectrum of skeletal muscle fiber types is found in adult skeletal muscles. The two major skeletal muscle fiber types are type I (slow-twitch) and type II (fast-twitch). Skeletal muscle type II fibers can be further subdivided into types IIa (fast red) and IIb (fast white).Synonyms: MLC-2, MLC-2A, MLC20, MRLC2, MYLC2B, Myosin regulatory light chain 2-B, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain MRLC2, SHUJUN-1, smooth muscle isoform

Molecular Weight: approx. 18 kDa

Gene ID: 103910

NCBI Accession: NP_001138416

UniProt: O14950

Pathways: Feeding Behaviour