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PAK1 antibody

PAK1 Reactivity: Human, Rat, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN272054
  • Target See all PAK1 Antibodies
    PAK1 (P21-Activated Kinase 1 (PAK1))
    Reactivity
    • 173
    • 94
    • 82
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 166
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 160
    • 13
    Polyclonal
    Conjugate
    • 90
    • 10
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    This PAK1 antibody is un-conjugated
    Application
    • 116
    • 66
    • 45
    • 39
    • 39
    • 33
    • 32
    • 14
    • 11
    • 10
    • 10
    • 6
    • 3
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects endogenous levels of PAK1 protein. (region surrounding Ile206)
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    Purity
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product PAK1 Primary Antibody
  • Application Notes
    ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should bedetermined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    DO NOT FREEZE!
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C.
  • Target
    PAK1 (P21-Activated Kinase 1 (PAK1))
    Alternative Name
    PAK1 (PAK1 Products)
    Synonyms
    X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody
    Background
    Pak1 belongs to the Ser/Thr protein kinase family and may function by modifying and partially stabilizing thermolabile DNA polymerases, perhaps during DNA repair. Expression of PAK1 increases specific activity of p53 in DNA-binding assays accompanied by a corresponding increase in transactivation. In vitro protein kinase assays show that GST-Pak1 can autophosphorylate, and can phosphorylate casein as an exogenous substrate. The Pak1 protein autophosphorylates on serine residues and preferentially binds to activated Cdc42p both in vitro and in vivo. This binding is mediated through the p21 binding domain on Pak1p and the effector domain on Cdc42p. Pak1 catalytic domain binds to the same highly conserved region on the regulatory domain that binds Cdc42, a GTPase protein capable of activating Pak1.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK
    Molecular Weight
    approx. 60 kDa
    Gene ID
    5058
    NCBI Accession
    NP_001122092
    UniProt
    Q13153
    Pathways
    MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis
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