PFKFB2 antibody
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- Target See all PFKFB2 Antibodies
- PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PFKFB2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of PFKFB2 protein. (region surrounding Pro479)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product PFKFB2 Primary Antibody
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- Application Notes
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))
- Alternative Name
- PFKFB2 (PFKFB2 Products)
- Synonyms
- pfkfb2 antibody, zgc:56282 antibody, PFK-2/FBPase-2 antibody, 4930568D07Rik antibody, RH2K antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 antibody, pfkfb2a antibody, PFKFB2 antibody, pfkfb2 antibody, Pfkfb2 antibody
- Background
- Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include, PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).Synonyms: 6-biphosphatase 2, 6-phosphofructo-2-kinase/fructose-2, PFK-2/FBPase-2, PFK/FBPase 2, PFKFB cardiac
- Molecular Weight
- approx. 58 kDa
- Gene ID
- 5208
- NCBI Accession
- NP_001018063
- UniProt
- O60825
- Pathways
- PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process
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