RPH3A antibody

Catalog No. ABIN272075

Product Details anti-RPH3A Antibody

Target: RPH3A (Rabphilin 3A (RPH3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Rabphilin 3A protein. (region surrounding Pro231)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: Rabphilin-3A / RPH3A (RPH3A Products)

Synonyms: 2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody

Background: The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B,and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A

Molecular Weight: approx. 75 kDa

Gene ID: 22895

NCBI Accession: NP_001137326

UniProt: Q9Y2J0

Pathways: Synaptic Vesicle Exocytosis