RHOA antibody

Catalog No. ABIN272117

Product Details anti-RHOA Antibody

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Rho A protein. (region surrounding Arg182)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Alternative Name: RhoA (RHOA Products)

Synonyms: ARH12 antibody, ARHA antibody, RHO12 antibody, RHOH12 antibody, Arha antibody, Arha1 antibody, Arha2 antibody, arha antibody, arha2 antibody, xrhoa antibody, arh12 antibody, rho12 antibody, rhoh12 antibody, rhoa antibody, RHO1 antibody, Rhoa antibody, wu:fa66g04 antibody, wu:fb26g07 antibody, zgc:66058 antibody, zgc:85805 antibody, ARHC antibody, rhoae antibody, zgc:64015 antibody, ras homolog family member A antibody, ras homolog family member A S homeolog antibody, ras homolog family member A L homeolog antibody, ras homolog gene family, member Aa antibody, ras homolog family member Ca antibody, RHOA antibody, Rhoa antibody, rhoa.S antibody, rhoa antibody, rhoa.L antibody, rhoaa antibody, rhoca antibody

Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1 (A and B), Rap 2, R-Ras and Ral (A and B), exhibit approximately 50 % amino acid homology to Ras. The three mammalian Rho proteins (A, B and C) are approximately 30 % homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine, A, aliphatic amino acid, X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.Synonyms: ARH12, ARHA, RHO12, Rho cDNA clone 12, Transforming protein RhoA

Molecular Weight: approx. 22 kDa

Gene ID: 387

NCBI Accession: NP_001655

UniProt: P61586

Pathways: Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome