SMAD1 antibody

Catalog No. ABIN272123

Product Details anti-SMAD1 Antibody

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Smad1 protein. (region surrounding Pro183)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Synonyms: MLP antibody, Xmad1 antibody, Xsmad1 antibody, bsp1 antibody, jv4-1 antibody, jv41 antibody, madh1 antibody, madr1 antibody, smad-1 antibody, MADH1 antibody, fb39h09 antibody, wu:fb39h09 antibody, wu:fb97e04 antibody, 2/23 antibody, CG12399 antibody, Dmel\\CG12399 antibody, E(zen)2 antibody, En(vvl) antibody, MAD antibody, Mat antibody, P-Mad antibody, P-mad antibody, PMad antibody, Smad antibody, Smad1 antibody, apg antibody, c28 antibody, dMAD antibody, dMad antibody, l(2)K00237 antibody, l(2)k00237 antibody, mad antibody, p-Mad antibody, pMAD antibody, pMad antibody, pSmad antibody, BSP-1 antibody, BSP1 antibody, JV4-1 antibody, JV41 antibody, MADR1 antibody, AI528653 antibody, Madh1 antibody, Madr1 antibody, Mlp1 antibody, MusMLP antibody, smad1 antibody, xmad antibody, SMAD family member 1 antibody, Mothers against dpp antibody, SMAD family member 1 L homeolog antibody, smad1 antibody, SMAD1 antibody, Mad antibody, Smad1 antibody, smad1.L antibody

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1

Molecular Weight: approx. 60 kDa

Gene ID: 4086

NCBI Accession: NP_001003688

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development