MED24 antibody

Catalog No. ABIN272143

Product Details anti-MED24 Antibody

Target: MED24 (Mediator Complex Subunit 24 (MED24))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED24 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of TRAP100 protein. (region surrounding Lys834)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MED24

Target: MED24 (Mediator Complex Subunit 24 (MED24))

Alternative Name: MED24 (MED24 Products)

Synonyms: CG7999 antibody, Dmel\\CG7999 antibody, Med24 antibody, Trap100 antibody, dTRAP100 antibody, l(3)psg5 antibody, MED24 antibody, THRAP4 antibody, DpseGA20755 antibody, dpse_GLEANR_1506 antibody, GA20755 antibody, dyak_GLEANR_4540 antibody, DyakGE20715 antibody, GE20715 antibody, dmoj_GLEANR_17526 antibody, DmojGI16770 antibody, GI16770 antibody, dper_GLEANR_4776 antibody, DperGL22535 antibody, GL22535 antibody, dsim_GLEANR_14081 antibody, DsimGD14060 antibody, GD14060 antibody, ARC100 antibody, CRSP100 antibody, CRSP4 antibody, DRIP100 antibody, TRAP100 antibody, 911GSE antibody, AU040102 antibody, AW547152 antibody, D11Ertd307e antibody, Gse2 antibody, Pparb2 antibody, Pparbp2 antibody, R75526 antibody, Thrap4 antibody, fc05c04 antibody, thrap4 antibody, trap100 antibody, wu:fc05c04 antibody, Mediator complex subunit 24 antibody, mediator complex subunit 24 antibody, mediator of RNA polymerase II transcription subunit 24 antibody, GA20755 gene product from transcript GA20755-RA antibody, DyakGE20715-PA antibody, GI16770 gene product from transcript GI16770-RA antibody, GL22535 gene product from transcript GL22535-RA antibody, GD14060 gene product from transcript GD14060-RA antibody, microRNA 6884 antibody, MED24 antibody, LOC100016544 antibody, LOC100398369 antibody, Dpse\Trap100 antibody, DyakTrap100 antibody, Dmoj\Trap100 antibody, Dper\Trap100 antibody, Dsim\Trap100 antibody, Med24 antibody, MIR6884 antibody, med24 antibody

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, and they are characterized by the presence of a nuclear receptor recognition motif, which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR-induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.Synonyms: ARC100, Activator-recruited cofactor 100 kDa component, CRSP complex 4, CRSP4, Cofactor required for Sp1 transcriptional activation subunit 4, DRIP100, KIAA0130, Mediator complex 24, Mediator of RNA polymerase II transcription subunit 24, THRAP4, TRAP100, Thyroid hormone receptor-associated protein 4, Thyroid hormone receptor-associated protein complex 100 kDa component, Vitamin D3 receptor-interacting protein complex component DRIP100

Molecular Weight: approx. 110 kDa

Gene ID: 9862

NCBI Accession: NP_001072986

UniProt: O75448

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity