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SUPT3H/SPT3 antibody

SUPT3H Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN272144
  • Target See all SUPT3H/SPT3 (SUPT3H) Antibodies
    SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))
    Reactivity
    • 25
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 24
    • 2
    Rabbit
    Clonality
    • 24
    • 2
    Polyclonal
    Conjugate
    • 14
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SUPT3H/SPT3 antibody is un-conjugated
    Application
    • 17
    • 13
    • 9
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of SPT3 protein. (region surrounding Glu203)
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    Purity
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product SUPT3H Primary Antibody
  • Application Notes
    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    DO NOT FREEZE!
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C.
  • Target
    SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))
    Alternative Name
    SUPT3H / SPT3 (SUPT3H Products)
    Synonyms
    SPT3 antibody, SPT3L antibody, supt3h antibody, MGC75923 antibody, zgc:92246 antibody, SUPT3H antibody, SPT3 homolog, SAGA and STAGA complex component antibody, SPT3 homolog, SAGA and STAGA complex component L homeolog antibody, SUPT3H antibody, supt3h antibody, supt3h.L antibody, Supt3h antibody
    Background
    The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.Synonyms: SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog
    Molecular Weight
    approx. 50 kDa
    Gene ID
    8464
    NCBI Accession
    NP_003590
    UniProt
    O75486
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