DGKI antibody

Catalog No. ABIN272146

Product Details anti-DGKI Antibody

Target: DGKI (Diacylglycerol Kinase, iota (DGKI))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKI antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of DGK-ι protein. (region surrounding Lys1023)

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DGKI

Target: DGKI (Diacylglycerol Kinase, iota (DGKI))

Alternative Name: DAG Kinase iota (DGKI Products)

Synonyms: DGK-IOTA antibody, rDGKi-3 antibody, C130010K08Rik antibody, diacylglycerol kinase iota antibody, diacylglycerol kinase, iota antibody, DGKI antibody, dgki antibody, LOC100560932 antibody, Dgki antibody

Background: Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and - γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-´(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-Ω (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-^θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DGKI, Diacylglycerol kinase iota

Molecular Weight: approx. 118 kDa

Gene ID: 9162

NCBI Accession: NP_004708

UniProt: O75912